Intrinsic repair of full-thickness articular cartilage defects in the axolotl salamander.
Intrinsic repair of full-thickness articular cartilage defects in the axolotl salamander.
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DOI：
10.1016/j.joca.2010.11.005
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发表时间：
2011-02
影响因子：
7
通讯作者：
MacLeod, J. N.
中科院分区：
文献类型：
--
作者：
Cosden, R. S.;Lattermann, C.;Romine, S.;Gao, J.;Voss, S. R.;MacLeod, J. N.
蝾螈（Ambystoma mexicanum）完全再生失去的肢体的能力使其成为研究组织再生的有价值的模型。目前的实验研究了这些脊椎动物修复大关节软骨缺损和恢复正常透明软骨和关节结构的能力，而不依赖于肢体截肢。通过切除股骨内侧髁至干骺端水平，造成全层关节软骨缺损。在术后0、2天、1、2、3、4、6、8、12、18、24、36和48周，在H & E和番红-O染色的7 μ m组织切片上分析修复过程。对称Kullback-Leibler（SKL）分歧用于评估蛋白多糖染色强度。对I型和II型胶原进行免疫组织化学。一个纤维的“interzone-like”组织占据的关节内空间的蝾螈股胫关节，没有证据表明，联合空洞观察。术后4周，缺损部位内的细胞表现出与间带样组织的细胞形态相似。在24周时，通过I型和II型胶原的免疫组织化学证实关节结构和软骨组织修复。番红-O染色的定量表明到18周蛋白多糖含量恢复。美西螈股胫关节的形态相似的发展哺乳动物diarthrodial关节。关节内间隙的细胞可能与间隙组织同源，有助于全层关节软骨缺损的内在修复。两者合计，这些结果表明，美西蝾螈可能作为一个有价值的模型，实现完全关节软骨修复的细胞和分子机制的调查。
The ability to fully regenerate lost limbs has made the axolotl salamander (Ambystoma mexicanum) a valuable model for studies of tissue regeneration. The current experiments investigate the ability of these vertebrates to repair large articular cartilage defects and restore normal hyaline cartilage and joint structure independent of limb amputation. Full-thickness articular cartilage defects were made by resection of the medial femoral condyle to the level of the metaphysis. At 0, 2 days, 1, 2, 3, 4, 6, 8, 12, 18, 24, 36 and 48 weeks post-surgery, the repair process was analyzed on H&E and Safranin-O stained 7 μm tissue sections. Symmetric Kullback–Leibler (SKL) divergences were used to assess proteoglycan staining intensities. Immunohistochemistry was performed for collagen types I and II. A fibrous “interzone-like” tissue occupies the intraarticular space of the axolotl femorotibial joint and no evidence of joint cavitation was observed. By 4 weeks post-surgery, cells within the defect site exhibited morphological similarities to those of the interzone-like tissue. At 24 weeks, joint structure and cartilaginous tissue repair were confirmed by immunohistochemistry for collagen types I and II. Quantitation of Safranin-O staining indicated restoration of proteoglycan content by 18 weeks. The axolotl femorotibial joint has morphological similarities to the developing mammalian diarthrodial joint. Cells in the intraarticular space may be homologous to the interzone tissue and contribute to intrinsic repair of full-thickness articular cartilage defects. Taken together, these results suggest that the axolotl may serve as a valuable model for the investigation of cellular and molecular mechanisms that achieve full articular cartilage repair.
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影响因子：
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作者：
Roy S;Lévesque M
通讯作者：
Lévesque M
DOI：
10.2106/00004623-198062010-00012
发表时间：
1980-01-01
影响因子：
5.3
作者：
FURUKAWA, T;EYRE, DR;GLIMCHER, MJ
通讯作者：
GLIMCHER, MJ
影响因子：
14
作者：
Jadin, Kyle D.;Bae, Won C.;Sah, Robert L.
通讯作者：
Sah, Robert L.
影响因子：
--
作者：
KULLBACK, S;LEIBLER, RA
通讯作者：
LEIBLER, RA
DOI：
10.1196/annals.1346.010
发表时间：
2006-01-01
期刊：
SKELETAL DEVELOPMENT AND REMODELING IN HEALTH, DISEASE, AND AGING
影响因子：
--
作者：
Pacifici, Maurizio;Koyama, Eiki;Iwamoto, Masahiro
通讯作者：
Iwamoto, Masahiro