Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators.
Bacterial community composition of chronic periodontitis and novel oral sampling sites for detecting disease indicators.
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DOI：
10.1186/2049-2618-2-32
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发表时间：
2014
期刊：
影响因子：
15.5
通讯作者：
Senadheera DB
中科院分区：
文献类型：
--
作者：
Galimanas V;Hall MW;Singh N;Lynch MD;Goldberg M;Tenenbaum H;Cvitkovitch DG;Neufeld JD;Senadheera DB
牙周炎是一种感染性和炎症性疾病的多微生物病因，可导致破坏骨骼和组织，支持牙齿。慢性牙周炎（CP）的管理在很大程度上依赖于消除或至少控制与疾病相关的已知致病性聚生体。到目前为止，从龈下（SubG）部位获得的微生物菌斑一直是使用深度测序进行细菌群落分析的主要焦点。除了SubG菌斑的使用，在这里，我们研究了从龈上（SupG）和舌背部位获得的菌斑是否可以作为监测CP相关细菌生物标志物的替代品。使用SubG，SupG，和舌斑DNA从11个健康和13个患病的受试者，我们测序V3区（约200个碱基）的16 S rRNA基因使用Illumina测序。在质量过滤之后，大约410万个序列被分解成操作分类单元（OTU;序列同一性截止值>97%），这些操作分类单元被分类为跨越114属的总共19门。细菌群落多样性和总体组成不受健康或疾病的影响，Bray-Curtis距离测量的多应答排列程序（MRPP）仅支持SubG和舌菌斑中细菌群落的弱差异，取决于健康或疾病状态（P < 0.05）。尽管如此，在SubG和舌部位，厚壁菌门的相对丰度从健康到疾病显著增加，并且在疾病的所有部位发现Synergistetes的成员丰度更高。在所有三个地点鉴定了指示CP的分类群（例如，齿垢密螺旋体、牙龈卟啉单胞菌、协同口腔分类群362和363）。这项研究首次表明，SupG和舌背斑块可以作为检测和计数已知和新的CP细菌生物标志物的替代来源。这一发现在临床上很重要，因为与需要训练有素的专业人员的SubG采样相比，从SupG和舌部位获得斑块方便且微创，并提供了一种在治疗结果监测期间跟踪CP生物标志物生物体的新方法。
Periodontitis is an infectious and inflammatory disease of polymicrobial etiology that can lead to the destruction of bones and tissues that support the teeth. The management of chronic periodontitis (CP) relies heavily on elimination or at least control of known pathogenic consortia associated with the disease. Until now, microbial plaque obtained from the subgingival (SubG) sites has been the primary focus for bacterial community analysis using deep sequencing. In addition to the use of SubG plaque, here, we investigated whether plaque obtained from supragingival (SupG) and tongue dorsum sites can serve as alternatives for monitoring CP-associated bacterial biomarkers. Using SubG, SupG, and tongue plaque DNA from 11 healthy and 13 diseased subjects, we sequenced V3 regions (approximately 200 bases) of the 16S rRNA gene using Illumina sequencing. After quality filtering, approximately 4.1 million sequences were collapsed into operational taxonomic units (OTUs; sequence identity cutoff of >97%) that were classified to a total of 19 phyla spanning 114 genera. Bacterial community diversity and overall composition was not affected by health or disease, and multiresponse permutation procedure (MRPP) on Bray-Curtis distance measures only supported weakly distinct bacterial communities in SubG and tongue plaque depending on health or disease status (P < 0.05). Nonetheless, in SubG and tongue sites, the relative abundance of Firmicutes was increased significantly from health to disease and members of Synergistetes were found in higher abundance across all sites in disease. Taxa indicative of CP were identified in all three locations (for example, Treponema denticola, Porphyromonas gingivalis, Synergistes oral taxa 362 and 363). For the first time, this study demonstrates that SupG and tongue dorsum plaque can serve as alternative sources for detecting and enumerating known and novel bacterial biomarkers of CP. This finding is clinically important because, in contrast with SubG sampling that requires trained professionals, obtaining plaque from SupG and tongue sites is convenient and minimally-invasive and offers a novel means to track CP-biomarker organisms during treatment outcome monitoring.
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影响因子：
11
作者：
Duran-Pinedo, Ana E.;Chen, Tsute;Frias-Lopez, Jorge
通讯作者：
Frias-Lopez, Jorge
影响因子：
4.4
作者：
Bartram, Andrea K.;Lynch, Michael D. J.;Neufeld, Josh D.
通讯作者：
Neufeld, Josh D.
影响因子：
9.4
作者：
Kumar, Purnima S.;Leys, Eugene J.;Griffen, Ann L.
通讯作者：
Griffen, Ann L.
影响因子：
3.7
作者：
Griffen AL;Beall CJ;Firestone ND;Gross EL;Difranco JM;Hardman JH;Vriesendorp B;Faust RA;Janies DA;Leys EJ
通讯作者：
Leys EJ
影响因子：
3.7
作者：
Liu B;Faller LL;Klitgord N;Mazumdar V;Ghodsi M;Sommer DD;Gibbons TR;Treangen TJ;Chang YC;Li S;Stine OC;Hasturk H;Kasif S;Segrè D;Pop M;Amar S
通讯作者：
Amar S