Deep sequencing of the oral microbiome reveals signatures of periodontal disease.
Deep sequencing of the oral microbiome reveals signatures of periodontal disease.
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DOI：
10.1371/journal.pone.0037919
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Amar S
中科院分区：
文献类型：
--
作者：
Liu B;Faller LL;Klitgord N;Mazumdar V;Ghodsi M;Sommer DD;Gibbons TR;Treangen TJ;Chang YC;Li S;Stine OC;Hasturk H;Kasif S;Segrè D;Pop M;Amar S
口腔微生物组是栖息在人类口腔中的微生物的复杂生态系统，含有数千种细菌类型。口腔内病原菌的增殖引起牙周炎，这是一种已知也构成心血管疾病的危险因素的炎性疾病。虽然对与发病机制相关的个体物种有很多了解，但对从健康到疾病转变的系统机制仍然知之甚少。通过对16S rRNA基因和整个社区DNA的测序，我们在15个龈下菌斑样本中与牙周炎相关的全球遗传、代谢和生态变化中提供了一个一瞥，其中两个牙周炎患者各有四个样本，其余样本来自三个健康个体。我们还展示了全宏基因组测序方法在表征口腔微生物组中关键参与者的基因组方面的能力，包括不可培养的TM7生物体。我们揭示了这种疾病的微生物组富含毒力因子，并适应了一种寄生的生活方式，这种生活方式利用了宿主体内平衡的破坏。此外，患病样本具有在完全健康样本中未发现的共同结构，这表明疾病状态可能占据口腔微生物组可能构型空间内的狭窄区域。我们的初步研究证明了高通量测序作为了解口腔微生物组在牙周病中作用的工具的力量。尽管测序水平不高（102道Illumina 76 bp PE）和人类DNA污染程度高（高达90%），但我们能够部分重建几种口腔微生物，并初步表征健康和患病口腔微生物组之间的一些系统水平差异。
The oral microbiome, the complex ecosystem of microbes inhabiting the human mouth, harbors several thousands of bacterial types. The proliferation of pathogenic bacteria within the mouth gives rise to periodontitis, an inflammatory disease known to also constitute a risk factor for cardiovascular disease. While much is known about individual species associated with pathogenesis, the system-level mechanisms underlying the transition from health to disease are still poorly understood. Through the sequencing of the 16S rRNA gene and of whole community DNA we provide a glimpse at the global genetic, metabolic, and ecological changes associated with periodontitis in 15 subgingival plaque samples, four from each of two periodontitis patients, and the remaining samples from three healthy individuals. We also demonstrate the power of whole-metagenome sequencing approaches in characterizing the genomes of key players in the oral microbiome, including an unculturable TM7 organism. We reveal the disease microbiome to be enriched in virulence factors, and adapted to a parasitic lifestyle that takes advantage of the disrupted host homeostasis. Furthermore, diseased samples share a common structure that was not found in completely healthy samples, suggesting that the disease state may occupy a narrow region within the space of possible configurations of the oral microbiome. Our pilot study demonstrates the power of high-throughput sequencing as a tool for understanding the role of the oral microbiome in periodontal disease. Despite a modest level of sequencing (∼2 lanes Illumina 76 bp PE) and high human DNA contamination (up to ∼90%) we were able to partially reconstruct several oral microbes and to preliminarily characterize some systems-level differences between the healthy and diseased oral microbiomes.
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影响因子：
2.1
作者：
Diaz-Torres, ML;Villedieu, A;Wilson, M
通讯作者：
Wilson, M
影响因子：
7.6
作者：
Barnes, V. M.;Teles, R.;Guo, L.
通讯作者：
Guo, L.
影响因子：
15.9
作者：
Cvitkovitch, DG;Liu, YH;Ellen, RP
通讯作者：
Ellen, RP
影响因子：
3.7
作者：
Ahn J;Yang L;Paster BJ;Ganly I;Morris L;Pei Z;Hayes RB
通讯作者：
Hayes RB
DOI：
10.1038/ismej.2010.30
发表时间：
2010-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
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