Polymer systems designed specifically for DNA sequencing by microchip electrophoresis: a comparison with commercially available materials.
Polymer systems designed specifically for DNA sequencing by microchip electrophoresis: a comparison with commercially available materials.
复制标题
DOI：
10.1002/elps.200800352
复制
发表时间：
2008-12
期刊：
影响因子：
2.9
通讯作者：
Barron, Annelise E.
中科院分区：
文献类型：
--
作者：
Fredlake, Christopher P.;Hert, Daniel G.;Root, Brian E.;Barron, Annelise E.
关键词：
基于电泳的DNA测序是唯一经过验证的大型复杂基因组从头测序技术。毛细管阵列电泳（CAE）仪器的小型化可以增加测序通量并降低成本，同时保持高质量和长读取长度，这使得CAE在从头测序中如此成功。专为微芯片测序平台设计的高性能聚合物基质和壁涂层的有限可用性仍然是成功开发商业微芯片测序仪器的主要障碍。一般认为，已经开发用于商业CAE仪器的基质和壁涂层将能够直接应用于微芯片装置，而测序性能几乎没有变化。在这里，我们表明，专门为微芯片电泳系统开发的测序矩阵可以在芯片上提供比一些最广泛使用的商业上可用的聚合物测序矩阵长150-300个碱基的读取长度。此外，我们表明，商业基质的涂层能力是在本研究中使用的硼硅酸盐芯片有效得多。这些结果导致的结论是，必须开发新的材料，使高性能的微型DNA测序仪器成为现实。
Electrophoresis-based DNA sequencing is the only proven technology for the de novo sequencing of large and complex genomes. Miniaturization of capillary array electrophoresis (CAE) instruments can increase sequencing throughput and decrease cost while maintaining the high quality and long read lengths that has made CAE so successful for de novo sequencing. The limited availability of high-performance polymer matrices and wall coatings designed specifically for microchip sequencing platforms continues to be a major barrier to the successful development of a commercial microchip sequencing instrument. It has been generally assumed that the matrices and wall coatings that have been developed for use in commercial CAE instruments will be able to be implemented directly into microchip devices with little to no change in sequencing performance. Here, we show that sequencing matrices developed specifically for microchip electrophoresis systems can deliver read lengths that are 150–300 bases longer on chip than some of the most widely used polymer sequencing matrices available commercially. Additionally, we show that the coating ability of commercial matrices is much less effective in the borosilicate chips used in this study. These results lead to the conclusion that new materials must be developed to make high-performance microfabricated DNA sequencing instruments a reality.
登录
查看更多内容
影响因子：
56.9
作者：
Gonzalez, E;Kulkarni, H;Ahuja, SK
通讯作者：
Ahuja, SK
影响因子：
2.9
作者：
Heller, C
通讯作者：
Heller, C
DOI：
10.1016/s0021-9673(01)95485-8
发表时间：
1985-01-01
期刊：
JOURNAL OF CHROMATOGRAPHY
影响因子：
--
作者：
HJERTEN, S
通讯作者：
HJERTEN, S
影响因子：
56.9
作者：
Gibbs, Richard A.;Rogers, Jeffrey;Zwieg, Ann S.
通讯作者：
Zwieg, Ann S.
DOI：
10.1073/pnas.0705093105
发表时间：
2008-01-15
影响因子：
11.1
作者：
Fredlake, Christopher P.;Hert, Daniel G.;Barron, Annelise E.
通讯作者：
Barron, Annelise E.