Optofluidic fluorescent imaging cytometry on a cell phone.
Optofluidic fluorescent imaging cytometry on a cell phone.
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DOI：
10.1021/ac201587a
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发表时间：
2011-09-01
影响因子：
7.4
通讯作者：
Ozcan, Aydogan
中科院分区：
文献类型：
--
作者：
Zhu, Hongying;Mavandadi, Sam;Coskun, Ahmet F.;Yaglidere, Oguzhan;Ozcan, Aydogan
荧光显微镜和流式细胞术是生物医学领域广泛使用的工具。将这些技术经济有效地转移到远程和资源有限的环境中可以创造新的机会，特别是对于远程医疗应用。朝着这个方向，我们在这里展示了使用紧凑、轻便且经济高效的光流控附件将成像细胞术和荧光显微镜集成在手机上。在这个基于手机的光流控成像细胞计数平台中，荧光标记的颗粒或感兴趣的细胞通过位于手机现有摄像头单元上方的一次性微流控通道连续输送到我们的成像体积。相同的微流体装置还充当多层光流体波导，并有效地引导我们的激发光，该激发光使用廉价的发光二极管从我们的微流体通道的侧面对接耦合。由于样品体积的激发是通过垂直于检测路径传播的导波发生的，因此我们的手机摄像头可以记录样品流过微通道时的荧光电影。然后快速处理这些荧光电影的数字帧，以量化感兴趣的目标溶液中标记颗粒/细胞的计数和密度。我们通过测量人体血液样本中白细胞的密度来测试基于手机的成像细胞仪的性能，这与市售血液分析仪相当匹配。我们进一步表征了同一平台的成像质量，以证明空间分辨率约为 2 μm。这种支持手机的光流控成像流式细胞仪尤其适用于快速、灵敏的体液成像，以进行各种细胞计数（例如，监测 HIV+ 患者）或稀有细胞分析，以及在偏远和资源匮乏的环境中筛查水质。
Fluorescent microscopy and flow cytometry are widely used tools in biomedical sciences. Cost-effective translation of these technologies to remote and resource-limited environments could create new opportunities especially for telemedicine applications. Toward this direction, here we demonstrate the integration of imaging cytometry and fluorescent microscopy on a cell phone using a compact, lightweight, and cost-effective optofluidic attachment. In this cell-phone-based optofluidic imaging cytometry platform, fluorescently labeled particles or cells of interest are continuously delivered to our imaging volume through a disposable microfluidic channel that is positioned above the existing camera unit of the cell phone. The same microfluidic device also acts as a multilayered optofluidic waveguide and efficiently guides our excitation light, which is butt-coupled from the side facets of our microfluidic channel using inexpensive light-emitting diodes. Since the excitation of the sample volume occurs through guided waves that propagate perpendicular to the detection path, our cell-phone camera can record fluorescent movies of the specimens as they are flowing through the microchannel. The digital frames of these fluorescent movies are then rapidly processed to quantify the count and the density of the labeled particles/cells within the target solution of interest. We tested the performance of our cell-phone-based imaging cytometer by measuring the density of white blood cells in human blood samples, which provided a decent match to a commercially available hematology analyzer. We further characterized the imaging quality of the same platform to demonstrate a spatial resolution of ~2 μm. This cell-phone-enabled optofluidic imaging flow cytometer could especially be useful for rapid and sensitive imaging of bodily fluids for conducting various cell counts (e.g., toward monitoring of HIV+ patients) or rare cell analysis as well as for screening of water quality in remote and resource-poor settings.
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影响因子：
6.1
作者：
Schmidt, Oliver;Bassler, Michael;Johnson, Noble
通讯作者：
Johnson, Noble
影响因子：
3.7
作者：
Smith ZJ;Chu K;Espenson AR;Rahimzadeh M;Gryshuk A;Molinaro M;Dwyre DM;Lane S;Matthews D;Wachsmann-Hogiu S
通讯作者：
Wachsmann-Hogiu S
DOI：
10.1111/j.1751-553x.2008.01093.x
发表时间：
2009-12-01
影响因子：
3
作者：
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通讯作者：
Lewis, S. M.
影响因子：
6.1
作者：
Tseng D;Mudanyali O;Oztoprak C;Isikman SO;Sencan I;Yaglidere O;Ozcan A
通讯作者：
Ozcan A
影响因子：
4.7
作者：
Hofmann-Wellenhof, R.;Salmhofer, W.;Soyer, H. P.
通讯作者：
Soyer, H. P.