MDA435/LCC6 and MDA435/LCC6MDR1: ascites models of human breast cancer.
MDA435/LCC6 and MDA435/LCC6MDR1: ascites models of human breast cancer.
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DOI：
10.1038/bjc.1996.29
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发表时间：
1996-01
影响因子：
8.8
通讯作者：
Clarke, R
中科院分区：
文献类型：
--
作者：
Leonessa, F;Green, D;Licht, T;Wright, A;WingateLegette, K;Lippman, J;Gottesman, MM;Clarke, R
我们已经建立了一种新的腹水肿瘤模型（MDA 435/LCC 6）从雌激素受体阴性，侵袭性和转移性MDA-MB-435人乳腺癌细胞系。MDA 435/LCC 6细胞在裸鼠和裸鼠体内以恶性腹水和实体瘤的形式生长，肿瘤发生率约为100%。未经处理的小鼠在腹膜内接种1 × 10（6）个细胞后产生腹水，可重复的寿命约为30天，所有动物均在48小时内死亡。MDA 435/LCC 6腹水对几种细胞毒性药物的体内反应，包括阿霉素、依托泊苷（VP-16）、BCNU和丝裂霉素C，密切反映了这些单一药物在先前未经治疗的乳腺癌患者中的活性。MDA 435/LCC 6细胞还保留了亲本MDA-MB-435细胞的贴壁依赖性和贴壁非依赖性体外生长特性，并且可用于标准体外药物筛选测定。MDA 435/LCC 6细胞的耐药模式表明它们可能具有很少的活性内源性耐药机制。为了生成用于筛选MDR 1逆转剂的模型，用指导MDR 1 cDNA组成型表达的逆转录病毒载体转导MDA 435/LCC 6，产生具有经典MDR 1耐药模式的细胞系（MDA 435/LCC 6 MDR 1）。这些腹水模型可能是小鼠白血病腹水（L1210，P388）的一种可行的替代方法，并与其他乳腺癌细胞系结合，促进新的细胞毒性药物和药物组合的体外和体内筛选。
We have established a novel ascites tumour model (MDA435/LCC6) from the oestrogen receptor-negative, invasive and metastatic MDA-MB-435 human breast cancer cell line. MDA435/LCC6 cells grow as both malignant ascites and solid tumours in vivo in nude mice and nude rats, with a tumour incidence of approximately 100%. Untreated mice develop ascites following i.p. inoculation of 1 x 10(6) cells and have a reproducible life span of approximately 30 days, with all animals dying within a 48 h period. The in vivo response of MDA435/LCC6 ascites to several cytotoxic drugs, including doxorubicin, etoposide (VP-16), BCNU and mitomycin C, closely reflects the activity of these single agents in previously untreated breast cancer patients. MDA435/LCC6 cells also retain the anchorage-dependent and anchorage-independent in vitro growth properties of the parental MDA-MB-435 cells, and can be used in standard in vitro drug screening assays. The drug resistance pattern of the MDA435/LCC6 cells suggests that they may have few active endogenous drug resistance mechanisms. To generate a model for the screening of MDR1-reversing agents, MDA435/LCC6 were transduced with a retroviral vector directing the constitutive expression of the MDR1 cDNA, producing a cell line with a classical MDR1 resistance pattern (MDA435/LCC6MDR1). THese ascites models may be a viable alternative to the murine leukaemia ascites (L1210, P388) and, in conjunction with other breast cancer cell lines, facilitate the in vitro and in vivo screening of new cytotoxic drugs and drug combinations.
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DOI：
10.1093/jnci/53.3.661
发表时间：
1974-01-01
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
CAILLEAU, R;YOUNG, R;REEVES, WJ
通讯作者：
REEVES, WJ
影响因子：
50.5
作者：
GOLDMAN, CA;SKINNIDER, LF;MAKSYMIUK, AW
通讯作者：
MAKSYMIUK, AW
影响因子：
--
作者：
BOTTI, G;CHIAPPETTA, G;CASCIONE, F
通讯作者：
CASCIONE, F
影响因子：
4
作者：
CLARKE, R;BRUNNER, N;LIPPMAN, ME
通讯作者：
LIPPMAN, ME
DOI：
10.1093/jnci/84.19.1506
发表时间：
1992-10-07
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
CLARKE, R;CURRIER, S;DICKSON, RB
通讯作者：
DICKSON, RB