Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157
Hybridization of strains of Escherichia coli O157 with probes derived from the eaeA gene of enteropathogenic E. coli and the eaeA homolog from a Vero cytotoxin-producing strain of E. coli O157
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大肠杆菌 O157 菌株与源自肠致病性大肠杆菌的 eaeA 基因和来自产生 Vero 细胞毒素的大肠杆菌 O157 菌株的 eaeA 同源物的探针杂交
DOI：
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发表时间：
1994
影响因子：
9.4
通讯作者：
Bernard Rowe
中科院分区：
文献类型：
--
作者：
G. Willshaw;S. Scotland;H. Smith;T. Cheasty;A. Thomas;Bernard Rowe
以经典致病性大肠杆菌eaeA基因中部的eae为探针，对375株大肠杆菌O 157进行菌落杂交。大肠杆菌E2348/69。用Vero细胞毒素（VT）产生菌的eae基因同源物C末端的探针eaeO 157对它们进行了检测。大肠杆菌（VTEC）O 157：H7血清型。两种探针均与所有246株O 157：H7或H-VTEC菌株杂交。大多数来自人类感染，其余来自牛。另外10株（H7或H-）与eae和eaeO 157序列杂交，但不与VT探针杂交。它们类似于O 157 VTEC，可能是天然存在的衍生物，失去了VT基因。其余119株O 157来自人类、动物和食物来源，属于H7以外的16种H型或H-型。它们是VT阴性的，并且在它们的性质上与O 157 VTEC不同：通常它们在1天内发酵山梨糖醇，产生β-葡萄糖醛酸酶，并且不能通过O 157 VTEC的方案进行噬菌体分型。eae探针而不是eaeO 157序列与18株H8或H39菌株杂交，主要来自人类腹泻。其余101株VT阴性菌株均不与探针杂交。而16株O 157：H45与一种弥漫粘附的E.大肠杆菌中表达，并以弥散方式附着于HEp-2细胞。血清群O 157包含具有异质性质的菌株。eaeO 157探针是用于区分O 157菌株的VT探针的有价值的补充。
A total of 375 Escherichia coli O157 strains were tested by colony hybridization with the eae probe from the central portion of the eaeA gene of the classical enteropathogenic E. coli strain E2348/69. They were also tested with a probe, eaeO157, from the C-terminal end of the eae gene homolog from a Vero cytotoxin (VT)-producing strain of E. coli (VTEC) of serotype O157:H7. Both probes hybridized with all 246 O157:H7 or H- VTEC strains tested. The majority were from human infections, and the remainder were from cattle. A further 10 strains (H7 or H-) hybridized with both eae and eaeO157 sequences but not with VT probes. They resembled O157 VTEC and were probably naturally occurring derivatives that had lost VT genes. The remaining 119 strains of O157 were from human, animal, and food sources and belonged to 16 H types other than H7 or were H-. They were VT negative and differed in their properties from O157 VTEC: generally they fermented sorbitol in 1 day, produced beta-glucuronidase, and could not be phage typed by the scheme for O157 VTEC. The eae probe but not the eaeO157 sequence hybridized with 18 H8 or H39 strains, predominantly from human diarrhea. The remaining 101 VT-negative strains hybridized with neither probe. However, 16 strains of O157:H45 hybridized with a probe for diffusely adherent E. coli and attached to HEp-2 cells in a diffuse pattern. Serogroup O157 comprises strains with heterogeneous properties. The eaeO157 probe is a valuable addition to the VT probes used to differentiate O157 strains.
影响因子：
6.4
作者：
BAUDRY, B;SAVARINO, SJ;LEVINE, MM
通讯作者：
LEVINE, MM
DOI：
10.1073/pnas.87.20.7839
发表时间：
1990-10-01
影响因子：
11.1
作者：
JERSE, AE;YU, J;KAPER, JB
通讯作者：
KAPER, JB
影响因子：
15.9
作者：
DONNENBERG, MS;TZIPORI, S;KAPER, JB
通讯作者：
KAPER, JB