Complementary RNA-Sequencing Based Transcriptomics and iTRAQ Proteomics Reveal the Mechanism of the Alleviation of Quinclorac Stress by Salicylic Acid in Oryza sativa ssp. japonica.
Complementary RNA-Sequencing Based Transcriptomics and iTRAQ Proteomics Reveal the Mechanism of the Alleviation of Quinclorac Stress by Salicylic Acid in Oryza sativa ssp. japonica.
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基于互补 RNA 测序的转录组学和 iTRAQ 蛋白质组学揭示水杨酸减轻粳稻二氯喹啉酸应激的机制
DOI：
10.3390/ijms18091975
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发表时间：
2017-09-14
影响因子：
5.6
通讯作者：
Zhou W
中科院分区：
文献类型：
--
作者：
Wang J;Islam F;Li L;Long M;Yang C;Jin X;Ali B;Mao B;Zhou W
为揭示水杨酸（SA）对水稻（Oryza sativa ssp）叶片醌胁迫的缓解机制。对经SA预处理和未经SA预处理的粳稻进行转录和蛋白质组学分析，分别确定差异表达基因（DEGs）和差异表达蛋白（DEPs）。结果表明，二氯丙酸胁迫改变了2207个DEPs（1427个上调，780个下调）和147个DEPs（98个下调，49个上调）的表达。这些基因和蛋白质富集于谷胱甘肽（GSH）代谢、卟啉和叶绿素代谢、次生代谢产物的生物合成、乙醛酸盐和二羧酸盐代谢等。它还影响无瓣a2-乙烯反应元件结合蛋白（AP2-EREBP）家族、成髓细胞病（MYB）家族和WRKY家族转录因子。经SA预处理后，697个基因和124个蛋白存在差异表达。通路分析显示谷胱甘肽、乙醛酸盐和二羧酸盐代谢相似的富集。转录因子分布于碱性螺旋-环-螺旋（bHLH）、MYB、Tify和WRKY家族。实时荧光定量PCR结果显示，喹诺酸胁迫诱导谷胱甘肽还原酶（GR）基因OsGR2、OsGR3的表达，经SA预处理进一步证实。Quinclorac胁迫进一步介导了水稻中乙醛的积累，而SA增强了OsALDH2B5和OsALDH7的表达，加速了除草剂Quinclorac的代谢，起到了保护水稻的作用。转录组学和蛋白质组学的相关分析表明，在喹诺酮胁迫下，相关蛋白/基因主要参与抑制叶绿素生物合成的中间步骤。根据转录组学和蛋白质组学的结果，讨论了除草剂喹诺酸调控和SA预处理调控的其他有趣的蛋白/基因和途径。
To uncover the alleviation mechanism of quinclorac stress by salicylic acid (SA), leaf samples of Oryza sativa ssp. Japonica under quinclorac stress with and without SA pre-treatment were analyzed for transcriptional and proteomic profiling to determine the differentially expressed genes (DEGs) and proteins (DEPs), respectively. Results showed that quinclorac stress altered the expression of 2207 DEGs (1427 up-regulated, 780 down-regulated) and 147 DEPs (98 down-regulated, 49 up-regulated). These genes and proteins were enriched in glutathione (GSH) metabolism, porphyrin and chlorophyll metabolism, the biosynthesis of secondary metabolites, glyoxylate and dicarboxylate metabolism, and so on. It also influenced apetala2- ethylene-responsive element binding protein (AP2-EREBP) family, myeloblastosis (MYB) family and WRKY family transcription factors. After SA pre-treatment, 697 genes and 124 proteins were differentially expressed. Pathway analysis showed similar enrichments in GSH, glyoxylate and dicarboxylate metabolism. Transcription factors were distributed in basic helix-loop-helix (bHLH), MYB, Tify and WRKY families. Quantitative real-time PCR results revealed that quinclorac stress induced the expression of glutathion reductase (GR) genes (OsGR2, OsGR3), which was further pronounced by SA pre-treatment. Quinclorac stress further mediated the accumulation of acetaldehyde in rice, while SA enhanced the expression of OsALDH2B5 and OsALDH7 to accelerate the metabolism of herbicide quinclorac for the protection of rice. Correlation analysis between transcriptome and proteomics demonstrated that, under quinclorac stress, correlated proteins/genes were mainly involved in the inhibition of intermediate steps in the biosynthesis of chlorophyll. Other interesting proteins/genes and pathways regulated by herbicide quinclorac and modulated by SA pre-treatment were also discussed, based on the transcriptome and proteomics results.
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影响因子：
3.7
作者：
Jisha V;Dampanaboina L;Vadassery J;Mithöfer A;Kappara S;Ramanan R
通讯作者：
Ramanan R
影响因子：
4.6
作者：
Islam F;Farooq MA;Gill RA;Wang J;Yang C;Ali B;Wang GX;Zhou W
通讯作者：
Zhou W
影响因子：
5.8
作者：
Gill, Rafaqat A.;Zhang, Na;Zhou, Weijun
通讯作者：
Zhou, Weijun
影响因子：
2.5
作者：
Grossmann, K
通讯作者：
Grossmann, K
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影响因子：
6.5
作者：
Cui, Meng;Zhang, Wenjiao;Wu, Ray
通讯作者：
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