Urinary Alpha-1-Acid Glycoprotein Is a Sensitive Marker of Glomerular Protein Leakage at Altitude.
Urinary Alpha-1-Acid Glycoprotein Is a Sensitive Marker of Glomerular Protein Leakage at Altitude.
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DOI：
10.1089/ham.2018.0017
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发表时间：
2018-09
影响因子：
2.1
通讯作者：
Bradwell AR
中科院分区：
文献类型：
--
作者：
Talks BJ;Bradwell SB;Delamere J;Rayner W;Clarke A;Lewis CT;Thomas OD;Bradwell AR
说吧本·J苏西B。放大图片作者：布拉德韦尔，约翰德拉米尔，威尔雷纳，亚历克斯克拉克，克里斯T.放大图片作者：刘易斯托马斯和亚瑟R.布拉德韦尔。尿α-1-酸性糖蛋白是高海拔地区肾小球蛋白渗漏的敏感标志物。High Alt Med Biol. 19：295-298，2018.-蛋白尿是上升到海拔高度的一个既定特征，可能是由于肾小球毛细血管壁（GCW）上的负电荷丢失引起的。为了验证这一假设，我们测量了尿液中两种大小相似但带相反电荷的蛋白质：带负电荷的α-1-酸性糖蛋白（α1-AGP，41-43 kDa）和带正电荷的二聚体λ游离轻链（λ-FLC，50 kDa）。将24小时尿漏与白蛋白（一种66 kDa带负电荷的蛋白质）进行比较。我们研究了23个人（年龄23-78岁，男性= 17）在基线（140米），每天在远征5035米。结果显示，随着海拔升高，α1-AGP（p < 0.0001; 6.85倍）和白蛋白（p = 0.0006; 1.65倍）的中位尿渗漏量显著增加，但λ-FLC的渗漏量无显著增加（p = 0.39; 1.14倍）。α1-AGP与每日上升曲线（p = 0.0026）和氧分压（p = 0.01）相关，而白蛋白无相关性（p = 0.19）。尿α1-AGP是一个比尿白蛋白和λ-FLC更敏感的高原蛋白尿标志物，并支持在高原GCW负电荷丢失的可能性。
Talks, Ben J., Susie B. Bradwell, John Delamere, Will Rayner, Alex Clarke, Chris T. Lewis, Owen D. Thomas, and Arthur R. Bradwell. Urinary alpha-1-acid glycoprotein is a sensitive marker of glomerular protein leakage at altitude. High Alt Med Biol. 19:295–298, 2018.—Proteinuria is an established feature of ascent to altitude and may be caused by a loss of negative charges on glomerular capillary walls (GCWs). To test this hypothesis, we measured two similar sized but oppositely charged proteins in urine: negatively charged alpha-1-acid glycoprotein (α1-AGP, 41–43 kDa) and positively charged dimeric lambda free light chains (λ-FLCs, 50 kDa). Twenty-four-hour urinary leakage was compared with albumin, a 66 kDa negatively charged protein. We studied 23 individuals (ages 23–78 years, male = 17) at baseline (140 m) and daily during an expedition to 5035 m. The results showed a significant increase in median urinary leakage of α1-AGP (p < 0.0001; 6.85-fold) and albumin (p = 0.0006; 1.65-fold) with ascent to altitude, but no significant increase in leakage of λ-FLCs (p = 0.39; 1.14-fold). α1-AGP correlated with the daily ascent profile (p = 0.0026) and partial pressure of oxygen (p = 0.01), whereas albumin showed no correlation (p = 0.19). Urinary α1-AGP was a more sensitive marker of altitude proteinuria than urinary albumin and λ-FLCs, and supported the possibility of loss of GCW negative charges at altitude.
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影响因子：
3.2
作者：
Jarad G;Miner JH
通讯作者：
Miner JH
影响因子：
7.3
作者：
Sand KM;Bern M;Nilsen J;Noordzij HT;Sandlie I;Andersen JT
通讯作者：
Andersen JT
影响因子：
5.1
作者：
WINTERBORN, MH;BRADWELL, AR;JONES, GT
通讯作者：
JONES, GT
影响因子：
--
作者：
Lewis, Christopher T.;Malein, W. L.;Clarke, S.
通讯作者：
Clarke, S.