Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi.
Differential expression of three members of the multidomain adhesion CCp family in Babesia bigemina, Babesia bovis and Theileria equi.
复制标题
DOI：
10.1371/journal.pone.0067765
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Knowles DP
中科院分区：
文献类型：
--
作者：
Bastos RG;Suarez CE;Laughery JM;Johnson WC;Ueti MW;Knowles DP
CCp蛋白家族的成员先前已被描述为在顶复门疟原虫寄生虫的配子体上表达。敲除疟原虫CCp基因可以阻断蚊子载体中寄生虫的发育，使CCp蛋白成为开发传播阻断疫苗的潜在靶点。牛巴贝斯虫和二联巴贝斯虫是牛巴贝斯虫病的病原体，马泰勒虫引起马梨形虫病。牛巴贝斯虫病和马梨形虫病分别是影响全球养牛业和马业的最重要的经济寄生虫病。B的最新测序。bovis和T.马基因组为鉴定寄生虫生物学中涉及的新基因提供了机会。在这里，我们描述了三个成员的CCp家族，命名为CCp 1，CCp 2和CCp 3，在B。二联胎，B. B. bovis和T.等。使用B。作为体外模型，在温度诱导的性阶段中证明了所有三种CCp基因和蛋白的表达。在T.在B期的血液中检测到CCp 3的转录物。牛而在T.马血液阶段或B。牛血液阶段或B。牛蜱阶段。总的来说，这些数据证明了B对多结构域粘附CCp家族的三个正向基因表达的差异模式。二联胎，B. B. bovis和T.等。新的CCp成员代表了控制牛巴贝斯虫病和马梨形虫病的创新方法的潜在靶点。
Members of the CCp protein family have been previously described to be expressed on gametocytes of apicomplexan Plasmodium parasites. Knocking out Plasmodium CCp genes blocks the development of the parasite in the mosquito vector, making the CCp proteins potential targets for the development of a transmission-blocking vaccine. Apicomplexans Babesia bovis and Babesia bigemina are the causative agents of bovine babesiosis, and apicomplexan Theileria equi causes equine piroplasmosis. Bovine babesiosis and equine piroplasmosis are the most economically important parasite diseases that affect worldwide cattle and equine industries, respectively. The recent sequencing of the B. bovis and T. equi genomes has provided the opportunity to identify novel genes involved in parasite biology. Here we characterize three members of the CCp family, named CCp1, CCp2 and CCp3, in B. bigemina, B. bovis and T. equi. Using B. bigemina as an in vitro model, expression of all three CCp genes and proteins was demonstrated in temperature-induced sexual stages. Transcripts for all three CCp genes were found in vivo in blood stages of T. equi, and transcripts for CCp3 were detected in vivo in blood stages of B. bovis. However, no protein expression was detected in T. equi blood stages or B. bovis blood stages or B. bovis tick stages. Collectively, the data demonstrated a differential pattern of expression of three orthologous genes of the multidomain adhesion CCp family by B. bigemina, B. bovis and T. equi. The novel CCp members represent potential targets for innovative approaches to control bovine babesiosis and equine piroplasmosis.
登录
查看更多内容
影响因子：
4
作者：
Mosqueda, J;Falcon, A;Figueroa, JV
通讯作者：
Figueroa, JV
影响因子：
56.9
作者：
Pain, A;Renauld, H;Hall, N
通讯作者：
Hall, N
影响因子：
4
作者：
Ferreri, Lucas M.;Brayton, Kelly A.;McElwain, Terry F.
通讯作者：
McElwain, Terry F.
影响因子：
2.6
作者：
Ribeiro, MFB;Patarroyo, JHS
通讯作者：
Patarroyo, JHS
影响因子：
15.3
作者：
Pradel, G;Hayton, K;Templeton, TJ
通讯作者：
Templeton, TJ