Meconium microbiome analysis identifies bacteria correlated with premature birth.
Meconium microbiome analysis identifies bacteria correlated with premature birth.
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DOI：
10.1371/journal.pone.0090784
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Neu J
中科院分区：
文献类型：
--
作者：
Ardissone AN;de la Cruz DM;Davis-Richardson AG;Rechcigl KT;Li N;Drew JC;Murgas-Torrazza R;Sharma R;Hudak ML;Triplett EW;Neu J
早产是全世界五岁以下儿童死亡的第二大原因，但许多病例的病因仍然是个谜。最近的研究结果对胎儿居住在无菌环境中的教条提出了挑战，并且出现了一个问题：胎儿定植的微生物是否可能与早产有关。有人认为胎便反映了子宫内的微生物环境。在这项研究中，检查了胎粪中的胎儿肠道细菌与胎龄之间的相关性，以提出可能导致早产的潜在机制。收集 52 名孕龄为 23 至 41 周的婴儿的胎便，提取 DNA，并进行 16S rRNA 分析。由此产生的微生物分类群与临床变量相关，并与之前对羊水和其他人类微生物群的研究进行比较。胎龄 <33 周的婴儿胎便中细菌 16S rRNA 的检出率有所增加。大约 61·1% 的测序读数被分类为在羊水中报告的属。孕龄对微生物群落结构的影响最大（R = 0·161；p = 0·029），而分娩方式（剖腹产与阴道分娩）也有影响（R = 0·100；p = 0·044）。肠杆菌属、肠球菌属、乳杆菌属、发光杆菌属和坦纳氏菌属与胎龄呈负相关，并且据报道会引起炎症反应，表明其在早产中具有致病作用。这提供了第一个证据来支持以下假设：源自吞咽羊水的胎儿肠道微生物群可能参与导致早产的炎症反应。
Preterm birth is the second leading cause of death in children under the age of five years worldwide, but the etiology of many cases remains enigmatic. The dogma that the fetus resides in a sterile environment is being challenged by recent findings and the question has arisen whether microbes that colonize the fetus may be related to preterm birth. It has been posited that meconium reflects the in-utero microbial environment. In this study, correlations between fetal intestinal bacteria from meconium and gestational age were examined in order to suggest underlying mechanisms that may contribute to preterm birth. Meconium from 52 infants ranging in gestational age from 23 to 41 weeks was collected, the DNA extracted, and 16S rRNA analysis performed. Resulting taxa of microbes were correlated to clinical variables and also compared to previous studies of amniotic fluid and other human microbiome niches. Increased detection of bacterial 16S rRNA in meconium of infants of <33 weeks gestational age was observed. Approximately 61·1% of reads sequenced were classified to genera that have been reported in amniotic fluid. Gestational age had the largest influence on microbial community structure (R = 0·161; p = 0·029), while mode of delivery (C-section versus vaginal delivery) had an effect as well (R = 0·100; p = 0·044). Enterobacter, Enterococcus, Lactobacillus, Photorhabdus, and Tannerella, were negatively correlated with gestational age and have been reported to incite inflammatory responses, suggesting a causative role in premature birth. This provides the first evidence to support the hypothesis that the fetal intestinal microbiome derived from swallowed amniotic fluid may be involved in the inflammatory response that leads to premature birth.
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影响因子：
7.2
作者：
Skogstrand, Kristin;Hougaard, David M.;Thorsen, Poul
通讯作者：
Thorsen, Poul
DOI：
10.1136/fetalneonatal-2011-301373
发表时间：
2012-11
期刊：
Archives of disease in childhood. Fetal and neonatal edition
影响因子：
--
作者：
Madan JC;Salari RC;Saxena D;Davidson L;O'Toole GA;Moore JH;Sogin ML;Foster JA;Edwards WH;Palumbo P;Hibberd PL
通讯作者：
Hibberd PL
影响因子：
9.8
作者：
Jarjoura, K;Devine, PC;Papapanou, PN
通讯作者：
Papapanou, PN
影响因子：
9.8
作者：
Onderdonk, Andrew B.;Hecht, Jonathan L.;Leviton, Alan
通讯作者：
Leviton, Alan
DOI：
10.1073/pnas.1002601107
发表时间：
2010-06-29
影响因子：
11.1
作者：
Dominguez-Bello, Maria G.;Costello, Elizabeth K.;Knight, Rob
通讯作者：
Knight, Rob