An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti.
An annotated catalogue of salivary gland transcripts in the adult female mosquito, Aedes aegypti.
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成年雌性埃及伊蚊唾液腺转录本的注释目录。
DOI：
10.1186/1471-2164-8-6
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发表时间：
2007-01-04
期刊：
影响因子：
4.4
通讯作者：
Wikel, Stephen K.
中科院分区：
文献类型：
--
作者：
Ribeiro, Jose M. C.;Arca, Bruno;Lombardo, Fabrizio;Calvo, Eric;Phan, Van My;Chandra, Prafulla K.;Wikel, Stephen K.
吸血节肢动物的唾液含有抗止血剂和免疫调节剂的混合物，有助于血液喂养。蚊子还以糖为食，其腺体的特殊区域含有糖苷酶和抗菌剂，可能有助于控制摄入食物中的细菌生长。为了扩展我们对埃及蚊（登革热和黄热病载体）唾液混合物的了解，我们分析了来自成年雌性蚊子 cDNA 文库的一组 4,232 个表达序列标签。描述了 614 个转录本（其中 573 个是新的）的非冗余目录，其中包括 136 个编码假定分泌性质的蛋白质。此外，对唾液腺 (SG) 匀浆进行二维凝胶电泳，然后对选定的蛋白质条带进行胰蛋白酶消化，MS/MS 分析揭示了 24 种蛋白质的表达。对这些基因子集的组织特异性转录的分析揭示了至少 31 个基因在女性 SG 中表达特异或丰富，而另外 24 个基因在女性 SG 和男性中表达，但在其他女性组织中不表达。这些 SG 转录本编码的 55 种蛋白质中的大多数没有已知的功能，并且代表了作为抗止血剂或抗菌剂进行表达和功能分析的高优先级候选者。一个意外的发现是出现了四个 SG 特有的蛋白质家族，它们可能是从原核生物水平转移到蚊子的产物。总体而言，本文贡献了 573 个新转录本的新颖鉴定，占 Æ 的近 3%。埃及蛋白质组假设有 20,000 个蛋白质组，并且是任何吸血昆虫中描述最清楚的唾液酸组。
Saliva of blood-sucking arthropods contains a cocktail of antihemostatic agents and immunomodulators that help blood feeding. Mosquitoes additionally feed on sugar meals and have specialized regions of their glands containing glycosidases and antimicrobials that might help control bacterial growth in the ingested meals. To expand our knowledge on the salivary cocktail of Ædes ægypti, a vector of dengue and yellow fevers, we analyzed a set of 4,232 expressed sequence tags from cDNA libraries of adult female mosquitoes. A nonredundant catalogue of 614 transcripts (573 of which are novel) is described, including 136 coding for proteins of a putative secretory nature. Additionally, a two-dimensional gel electrophoresis of salivary gland (SG) homogenates followed by tryptic digestion of selected protein bands and MS/MS analysis revealed the expression of 24 proteins. Analysis of tissue-specific transcription of a subset of these genes revealed at least 31 genes whose expression is specific or enriched in female SG, whereas 24 additional genes were expressed in female SG and in males but not in other female tissues. Most of the 55 proteins coded by these SG transcripts have no known function and represent high-priority candidates for expression and functional analysis as antihemostatic or antimicrobial agents. An unexpected finding is the occurrence of four protein families specific to SG that were probably a product of horizontal transfer from prokaryotic organisms to mosquitoes. Overall, this paper contributes to the novel identification of 573 new transcripts, or near 3% of the Æ. ægypti proteome assuming a 20,000-protein set, and to the best-described sialome of any blood-feeding insect.
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DOI：
10.1073/pnas.91.1.138
发表时间：
1994-01-04
影响因子：
11.1
作者：
CHAMPAGNE, DE;RIBEIRO, JMC
通讯作者：
RIBEIRO, JMC
影响因子：
3.5
作者：
JAMES, AA;BLACKMER, K;RACIOPPI, JV
通讯作者：
RACIOPPI, JV
DOI：
10.1006/bbrc.2001.5675
发表时间：
2001-10-05
影响因子：
3.1
作者：
Hirai, M;Wang, JC;Matsuoka, H
通讯作者：
Matsuoka, H
影响因子：
9.5
作者：
Berriman, Matt;Rutherford, Kim
通讯作者：
Rutherford, Kim
影响因子：
64.8
作者：
Billker, O;Lindo, V;Morris, HR
通讯作者：
Morris, HR