The Extracts and Major Compounds Derived from Astragali Radix Alter Mitochondrial Bioenergetics in Cultured Cardiomyocytes: Comparison of Various Polar Solvents and Compounds.
The Extracts and Major Compounds Derived from Astragali Radix Alter Mitochondrial Bioenergetics in Cultured Cardiomyocytes: Comparison of Various Polar Solvents and Compounds.
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DOI：
10.3390/ijms19061574
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发表时间：
2018-05-25
影响因子：
5.6
通讯作者：
Tsim KWK
中科院分区：
文献类型：
--
作者：
Huang Y;Kwan KKL;Leung KW;Wang H;Kong XP;Dong TTX;Tsim KWK
黄芪是我国常用的补气药，具有强壮生物组织的滋补作用。AR提取物中含有丰富的抗氧化剂，包括黄芪皂苷和黄芪甲苷。然而，很少有报道系统地测量AR的主要成分对细胞线粒体生物能量学的影响。在这里，开发了一种采用细胞外通量分析仪的系统方法来评估培养的心肌细胞H9 C2中的线粒体呼吸。比较了不同极性提取物以及AR的主要化合物的作用。采用液相色谱-质谱联用仪（LC-MS）测定水、50%乙醇和90%乙醇提取物中的黄芪甲苷、毛蕊异黄酮、芒柄花素和染料木素的含量。AR提取物的抗氧化活性，以及它们的主要化合物，通过测量的自由基清除活性和保护作用，在叔丁基过氧化氢（TBHP）处理的H9 C2细胞。通过监测实时耗氧率（OCR）在tBHP处理的心肌细胞与海马细胞外通量分析仪，滋补作用的AR提取物和它们的主要化合物对线粒体生物能量学进行了评价。AR水提取物具有最强的抗氧化活性和保护作用的心肌细胞暴露于氧化应激。这种保护作用可能是通过增加应激细胞的备用呼吸能力和线粒体ATP生成来介导的。AR的主要化合物，黄芪甲苷IV和染料木素，在调节线粒体生物能量学方面表现出相反的作用。这些结果表明，高极性提取物的AR，特别是黄芪甲苷富集的提取物，具有更好的滋补作用的线粒体的培养心肌细胞的生物能量比提取物具有较低的极性。
Astragali Radix (AR) is a widely used “Qi-invigorating” herb in China for its tonic effects in strengthening biological tissues. The extract of AR contains abundant antioxidants, including astragalosides and isoflavonoids. However, very few reports have systematically measured the effects of the major components of AR on cell mitochondrial bioenergetics. Here, a systemic approach employing an extracellular flux analyzer was developed to evaluate mitochondrial respiration in cultured cardiomyocyte cells H9C2. The effects of different polar extractives, as well as of the major compounds of AR, were compared. The contents of astragaloside IV, calycosin, formononetin, and genistein in the AR extracts obtained by using water, 50% ethanol, and 90% ethanol were measured by liquid chromatograph-mass spectrometer (LC–MS). The antioxidant activities of the AR extracts, as well as of their major compounds, were determined by measuring the free radical scavenging activity and protective effects in tert-butyl hydroperoxide (tBHP)-treated H9C2 cells. By monitoring the real-time oxygen consumption rate (OCR) in tBHP-treated cardiomyocytes with a Seahorse extracellular flux analyzer, the tonic effects of the AR extracts and of their main compounds on mitochondrial bioenergetics were evaluated. AR water extracts possessed the strongest antioxidant activity and protective effects in cardiomyocytes exposed to oxidative stress. The protection was proposed to be mediated via increasing the spare respiratory capacity and mitochondrial ATP production in the stressed cells. The major compounds of AR, astragaloside IV and genistein, showed opposite effects in regulating mitochondrial bioenergetics. These results demonstrate that highly polar extracts of AR, especially astragaloside-enriched extracts, possess better tonic effects on mitochondrial bioenergetics of cultured cardiomyocytes than extracts with a lower polarity.
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影响因子：
7.9
作者：
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DOI：
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发表时间：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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