Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus.
Comparison of enzyme-linked immunosorbent assay and RT-PCR for the detection of porcine epidemic diarrhoea virus.
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DOI：
10.1016/j.rvsc.2009.05.009
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发表时间：
2010-03
影响因子：
2.4
通讯作者：
Cordioli P
中科院分区：
文献类型：
--
作者：
Sozzi E;Luppi A;Lelli D;Martin AM;Canelli E;Brocchi E;Lavazza A;Cordioli P
猪流行性腹泻(PED)是一种由冠状病毒引起的猪传染性肠道疾病。建立了检测猪流行性腹泻病毒(PEDV)的双抗体夹心酶联免疫吸附试验(DAS-EL ISA)。在2006-2007年间收集的506个猪样本中，DAS-ELISA与RT-PCR进行了比较，这些样本来自于来自蒲河谷不同农场的猪。这项研究包括直接从有临床症状的活体动物的直肠获取的粪便样本和从死亡猪的盲肠收集的肠道样本。两种方法检测粪便标本的相关性(K=0.97，95%CI：0.94~1.00)高于肠道样品的相关性(K=0.62，95%CI：0.35~0.89)。ELISA技术的使用为评估现场样本中冠状病毒PED抗原的存在提供了一种有效的手段，并表明该方法在流行病学研究中是一种非常有用的工具。
Porcine epidemic diarrhoea (PED) is a contagious enteric disease of pigs caused by a coronavirus. A double antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) based on the use of monoclonal antibodies was developed for the detection of porcine epidemic diarrhoea virus (PEDV). The DAS-ELISA was compared with RT-PCR in the examination of 506 specimens collected during 2006–2007 from pigs originating from different farms located in the Po valley. Both faecal samples obtained directly from the rectum of live animals showing clinical signs and intestinal samples collected from the caecum of deceased pigs were included in the study. The correlation between the two methods was higher when testing faecal samples (K = 0.97, 95% CI: 0.94–1.00) than testing intestinal samples (K = 0.62, 95% CI: 0.35–0.89). The use of ELISA technology provided an efficient and effective mean of evaluating the presence of coronavirus PED antigen in field samples and indicates that this procedure is a very useful tool in epidemiological studies.
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