Effects of physiological electric fields on migration of human dermal fibroblasts.
Effects of physiological electric fields on migration of human dermal fibroblasts.
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DOI：
10.1038/jid.2010.96
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发表时间：
2010-09
影响因子：
6.5
通讯作者：
Zhao, Min
中科院分区：
文献类型：
--
作者：
Guo, Aihua;Song, Bing;Reid, Brian;Gu, Yu;Forrester, John V.;Jahoda, Colin A. B.;Zhao, Min
皮肤伤口处立即产生的内源电流引导上皮细胞迁移，并且可能对伤口愈合很重要。成纤维细胞的迁移对于伤口愈合至关重要。目前尚不清楚伤口电场如何引导真皮成纤维细胞的迁移。我们在此报道，小鼠皮肤伤口会产生内源电流长达数小时。原代培养物和细胞系培养物的人真皮成纤维细胞在 50-100 mV mm−1 的电场中定向但缓慢地向阳极迁移。这与角质形成细胞不同，角质形成细胞快速迁移到阴极。真皮成纤维细胞需要1个多小时才能表现出可检测到的定向迁移。需要更大的场强（400 mV mm−1）才能在场开始后 1 小时内诱导定向迁移。磷脂酰肌醇-3-OH 激酶（PI3 激酶）介导角质形成细胞的阴极定向迁移。我们测试了 PI3 激酶在成纤维细胞阳极定向迁移中的作用。施加的电场激活真皮成纤维细胞中的 PI3 激酶/Akt。 p110γ（一种 PI3 激酶催化亚基）无效小鼠的真皮成纤维细胞显示定向迁移显着减少。这些结果表明，生理电场可能以不同的方式调节真皮成纤维细胞和角质形成细胞的运动，尽管使用类似的 PI3 激酶依赖性机制。
Endogenous electric currents generated instantly at skin wounds direct migration of epithelial cells and are likely to be important in wound healing. Migration of fibroblasts is critical in wound healing. It remains unclear how wound electric fields guide migration of dermal fibroblasts. We report here that mouse skin wounds generated endogenous electric currents for many hours. Human dermal fibroblasts of both primary and cell-line cultures migrated directionally but slowly toward the anode in an electric field of 50–100 mV mm−1. This is different from keratinocytes, which migrate quickly to the cathode. It took more than 1 hour for dermal fibroblasts to manifest detectable directional migration. Larger field strength (400 mV mm−1) was required to induce directional migration within 1 hour after onset of the field. Phosphatidylinositol-3-OH kinase (PI3 kinase) mediates cathode-directed migration of keratinocytes. We tested the role of PI3 kinase in anode-directed migration of fibroblasts. An applied electric field activated PI3 kinase/Akt in dermal fibroblasts. Dermal fibroblasts from p110γ (a PI3 kinase catalytic subunit) null mice showed significantly decreased directional migration. These results suggest that physiological electric fields may regulate motility of dermal fibroblasts and keratinocytes differently, albeit using similar PI3 kinase-dependent mechanisms.
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影响因子：
--
作者：
GRULER, H;NUCCITELLI, R
通讯作者：
NUCCITELLI, R
影响因子：
7.8
作者：
Bandyopadhyay, Balaji;Fan, Jianhua;Guan, Shengxi;Li, Yong;Chen, Mei;Woodley, David T;Li, Wei
通讯作者：
Li, Wei
影响因子：
2.4
作者：
DAVIS, SC;OVINGTON, LG
通讯作者：
OVINGTON, LG
影响因子：
7.8
作者：
Erickson, C A;Nuccitelli, R
通讯作者：
Nuccitelli, R
影响因子：
4
作者：
Finkelstein, E;Chang, W;Bulinski, JC
通讯作者：
Bulinski, JC