Differential regulation of GLUT1 activity in human corneal limbal epithelial cells and fibroblasts.
Differential regulation of GLUT1 activity in human corneal limbal epithelial cells and fibroblasts.
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DOI：
10.1016/j.biochi.2012.09.022
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发表时间：
2013-02
期刊：
影响因子：
3.9
通讯作者：
Louters, Larry L.
中科院分区：
文献类型：
--
作者：
Kuipers, David P.;Scripture, Jared P.;Gunnink, Stephen M.;Salie, Matthew J.;Schotanus, Mark P.;Ubels, John L.;Louters, Larry L.
角膜上皮组织是一层快速生长的细胞，其高度糖酵解并表达作为主要葡萄糖转运蛋白的GLUT 1。研究表明，L929成纤维细胞和其他细胞系中的GLUT1可被多种试剂急性激活，但角膜细胞葡萄糖摄取的急性调节尚未得到系统研究。因此，我们研究了永生化的人角膜缘上皮（HCLE）细胞系中的葡萄糖摄取，并将其与L929成纤维细胞中的葡萄糖摄取进行了比较，L929成纤维细胞是葡萄糖摄取已被很好表征的细胞系。我们报告说，HCLE细胞中GLUT1的表达是L929成纤维细胞的6.6倍，但HCLE细胞的基础葡萄糖摄取率高25倍。用干扰线粒体代谢的试剂（如叠氮化钠和小檗碱）处理，在L929细胞中激活葡萄糖摄取超过3倍，但对葡萄糖摄取HCLE细胞没有影响。此外，已知与硫醇反应的试剂，如肉桂醛、氧化苯胂和硝酰基刺激L929细胞中的葡萄糖摄取3至4倍，但实际上抑制HCLE细胞中的葡萄糖摄取。这些数据表明，在快速生长的HCLE细胞中，GLUT1以更高的浓度表达，并且在基础条件下已经高度活化。这些数据支持GLUT 1急性激活的模型，该模型表明GLUT 1的活性通过GLUT 1自身内部二硫键的形成而增强。
The corneal epithelial tissue is a layer of rapidly growing cells that are highly glycolytic and express GLUT1 as the major glucose transporter. It has been shown that GLUT1 in L929 fibroblast cells and other cell lines can be acutely activated by a variety agents.However, the acute regulation of glucose uptake in corneal cells has not been systematically investigated. Therefore, we examined glucose uptake in an immortalized human corneal–limbal epithelial (HCLE) cell line and compared it to glucose uptake in L929 fibroblast cells, a cell line where glucose uptake has been well characterized. We report that the expression of GLUT1 in HCLE cells is 6.6-fold higher than in L929 fibroblast cells, but the HCLE cells have a 25-fold higher basal rate of glucose uptake. Treatment with agents that interfere with mitochondrial metabolism, such as sodium azide and berberine, activate glucose uptake in L929 cells over 3-fold, but have no effect on glucose uptake HCLE cells. Also, agents known to react with thiols, such cinnamaldehyde, phenyarsine oxide and nitroxyl stimulate glucose uptake in L929 cells 3 to 4-fold, but actually inhibit glucose uptake in HCLE cells. These data suggest that in the fast growing HCLE cells, GLUT1 is expressed at a higher concentration and is already highly activated at basal conditions. These data support a model for the acute activation of GLUT1 that suggests that the activity of GLUT1 is enhanced by the formation of an internal disulfide bond within GLUT1 itself.
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影响因子：
11.2
作者：
Furuta, Eiji;Okuda, Hiroshi;Kobayashi, Aya;Watabe, Kounosuke
通讯作者：
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影响因子：
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作者：
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