Effects of cinnamaldehyde on the glucose transport activity of GLUT1.
Effects of cinnamaldehyde on the glucose transport activity of GLUT1.
复制标题
DOI：
10.1016/j.biochi.2010.10.006
复制
发表时间：
2011-02
期刊：
影响因子：
3.9
通讯作者：
Louters, Larry L.
中科院分区：
文献类型：
--
作者：
Plaisier, Christina;Cok, Alexandra;Scott, Jordan;Opejin, Adeleye;Bushhouse, Kelsey T.;Salie, Mathew J.;Louters, Larry L.
越来越多的证据表明，肉桂提取物含有增强胰岛素作用的成分。然而，关于肉桂对非胰岛素刺激的葡萄糖摄取的影响知之甚少。因此，在基础条件和通过葡萄糖剥夺激活葡萄糖摄取的条件下，检查了肉桂醛对L929成纤维细胞中GLUT 1的葡萄糖转运活性的影响。数据显示，肉桂醛对GLUT 1的葡萄糖转运活性具有双重作用。在基础条件下，它刺激葡萄糖摄取，并达到3.5倍的最大刺激在2.0 mM。然而，肉桂醛也抑制葡萄糖摄取的激活葡萄糖剥夺以剂量依赖性的方式。肉桂醛类似物的实验表明，这些活性取决于肉桂醛中的α，β-不饱和醛结构基序。肉桂醛的抑制活性，但不是刺激活性保持后，清洗恢复期。用巯基化合物（如β-巯基乙醇或半胱氨酸）预处理肉桂醛可阻断肉桂醛的抑制活性。这些结果表明，肉桂醛通过与靶半胱氨酸残基形成共价连接来抑制GLUT 1的活化。肉桂醛对GLUT 1转运活性的这种双重活性表明肉桂酸不是肉桂抗糖尿病特性的主要贡献者。
There is accumulating evidence that cinnamon extracts contain components that enhance insulin action. However, little is know about the effects of cinnamon on non-insulin stimulated glucose uptake. Therefore, the effects of cinnamaldehyde on the glucose transport activity of GLUT1 in L929 fibroblast cells were examined under both basal conditions and conditions where glucose uptake is activated by glucose deprivation. The data reveal that cinnamaldehyde has a dual action on the glucose transport activity of GLUT1. Under basal conditions it stimulates glucose uptake and reaches a 3.5 fold maximum stimulation at 2.0 mM. However, cinnamaldehyde also inhibits the activation of glucose uptake by glucose deprivation in a dose dependent manner. Experiments with cinnamaldehyde analogs reveal that these activities are dependent on the α,β-unsaturated aldehyde structural motif in cinnamaldehyde. The inhibitory, but not the stimulatory activity of cinnamaldehyde was maintained after a wash-recovery period. Pretreatment of cinnamaldehyde with thiol-containing compounds, such as β-mercaptoethanol or cysteine, blocked the inhibitory activity of cinnamaldehyde. These results suggest that cinnamaldehyde inhibits the activation of GLUT1 by forming a covalent link to target cysteine residue/s. This dual activity of cinnamaldehyde on the transport activity of GLUT1 suggests that cinnamaldehye is not a major contributor to the anti-diabetic properties of cinnamon.
登录
查看更多内容
影响因子：
6.1
作者：
Peng, Xiaofang;Cheng, Ka-Wing;Wang, Mingfu
通讯作者：
Wang, Mingfu
影响因子：
16.2
作者：
Altschuler, Justin A.;Casella, Samuel J.;Curtis, Kevin M.
通讯作者：
Curtis, Kevin M.
影响因子：
2
作者：
Kim, So Hui;Shin, Eun-Jung;Hyun, Chang-Kee
通讯作者：
Hyun, Chang-Kee
影响因子：
--
作者：
HENRIKSEN, EJ;HOLLOSZY, JO
通讯作者：
HOLLOSZY, JO
DOI：
10.1080/07315724.2001.10719053
发表时间：
2001-08-01
影响因子：
3.5
作者：
Jarvill-Taylor, KJ;Anderson, RA;Graves, DJ
通讯作者：
Graves, DJ