Fluorescence-based assessment of plasma-induced hydrophilicity in microfluidic devices via Nile Red adsorption and depletion.
Fluorescence-based assessment of plasma-induced hydrophilicity in microfluidic devices via Nile Red adsorption and depletion.
复制标题
基于荧光的血浆诱导的微流体设备中的亲水性评估,通过尼罗红色吸附和耗竭评估。
DOI:
10.1021/ac501259n
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发表时间:
2014-08-05
影响因子:
7.4
通讯作者:
Beebe, David J.
中科院分区:
文献类型:
--
作者:
Guckenberger, David J.;Berthier, Erwin;Young, Edmond W. K.;Beebe, David J.
We present a simple method, called fluorescence-based assessment of plasma-induced hydrophilicity (FAPH), that enables spatial mapping of the local hydrophilicity of surfaces normally inaccessible by traditional contact angle measurement techniques. The method leverages the change in fluorescence of a dye, Nile Red, which is adsorbed on an oxygen plasma-treated surface, and its correlation with the contact angle of water. Using FAPH, we explored the effect of microchannel geometries on the penetration distance of oxygen plasma into a microchannel and found that entrance effects prevent uniform treatment. We showed that these variations have a significant impact on cell culture, and thus the design of cell-based microfluidic assays must consider this phenomenon to obtain repeatable and homogeneous results.
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影响因子:
2.7
作者:
Jo, BH;Van Lerberghe, LM;Beebe, DJ
通讯作者:
Beebe, DJ
DOI:
10.1016/1010-6030(95)04140-0
发表时间:
1996-01-04
影响因子:
4.3
作者:
Dutta, AK;Kamada, K;Ohta, K
通讯作者:
Ohta, K
DOI:
10.1016/j.msec.2012.12.081
发表时间:
2013-04-01
影响因子:
7.9
作者:
Yang, Seung Yun;Kim, Eung-Sam;Kim, Jin Ron
通讯作者:
Kim, Jin Ron
影响因子:
6.7
作者:
Lee, Chongmu;Kim, Hyoun Woo;Kim, Sookjoo
通讯作者:
Kim, Sookjoo
影响因子:
6.1
作者:
Riaz, Asif;Gandhiraman, Ram P.;Lee, Luke P.
通讯作者:
Lee, Luke P.