Fluorescence-based assessment of plasma-induced hydrophilicity in microfluidic devices via Nile Red adsorption and depletion.

Fluorescence-based assessment of plasma-induced hydrophilicity in microfluidic devices via Nile Red adsorption and depletion.
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基于荧光的血浆诱导的微流体设备中的亲水性评估,通过尼罗红色吸附和耗竭评估。

DOI:
10.1021/ac501259n
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发表时间:
2014-08-05
影响因子:
7.4
通讯作者:
Beebe, David J.
Beebe, David J.
中科院分区:
化学1区
文献类型:
--
作者:
Guckenberger, David J.;Berthier, Erwin;Young, Edmond W. K.;Beebe, David J.

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我们提出了一种简单的方法,称为基于荧光的等离子体诱导的亲水性(FAPH)的评估,使空间映射的局部亲水性的表面通常无法通过传统的接触角测量技术。该方法利用了染料尼罗红的荧光变化,该染料被吸附在氧等离子体处理的表面上,并且其与水的接触角相关。使用FAPH,我们探讨了微通道的几何形状上的氧等离子体进入微通道的渗透距离的影响,并发现入口效应阻止均匀处理。我们发现这些变化对细胞培养有显著影响,因此基于细胞的微流控分析的设计必须考虑这种现象,以获得可重复和均匀的结果。
We present a simple method, called fluorescence-based assessment of plasma-induced hydrophilicity (FAPH), that enables spatial mapping of the local hydrophilicity of surfaces normally inaccessible by traditional contact angle measurement techniques. The method leverages the change in fluorescence of a dye, Nile Red, which is adsorbed on an oxygen plasma-treated surface, and its correlation with the contact angle of water. Using FAPH, we explored the effect of microchannel geometries on the penetration distance of oxygen plasma into a microchannel and found that entrance effects prevent uniform treatment. We showed that these variations have a significant impact on cell culture, and thus the design of cell-based microfluidic assays must consider this phenomenon to obtain repeatable and homogeneous results.
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