Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood.
Fast, Sensitive, and Quantitative Point-of-Care Platform for the Assessment of Drugs of Abuse in Urine, Serum, and Whole Blood.
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快速，敏感和定量的护理平台，用于评估尿液，血清和全血的滥用药物。
DOI：
10.1021/acs.analchem.7b01288
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发表时间：
2017-08-15
影响因子：
7.4
通讯作者：
Qin L
中科院分区：
文献类型：
--
作者：
Li Y;Uddayasankar U;He B;Wang P;Qin L
药物滥用是欧洲和北美许多国家的一个主要公共卫生问题。目前，用于药物滥用评估的可用平台面临着非定量、不准确、低通量、与各种复杂样本不兼容、分析时间长以及读取所需的仪器和/或专业知识等技术挑战。在这里，我们报告了一个集成的竞争性体积条形图芯片（CV芯片），以测定多个药物靶点的护理点（POC）。据我们所知，这是第一次证明POC平台可以完全解决上述限制。我们应用这种集成的CV-Chip平台检测了38例患者尿液和血清样本中的多种药物，并使用LC-MS/MS方法验证了芯片结果，表明临床灵敏度和特异性分别为0.94和1.00。我们进一步证明，芯片上血液分离器与CV芯片的组合使该平台能够直接分析手指针刺全血样本，这一直被认为是POC检测的理想生物样本。总之，这种集成的CV芯片能够作为一个灵敏、准确、快速、便携、读出可视和微创的药物滥用评估平台。
Drug abuse is a major public health problem in many countries in Europe and North America. Currently available platforms for drug abuse assessment are facing technical challenges of non-quantitation, inaccuracy, low throughput, incompatible with diverse complex specimens, long assay time and requirement of instrument and/or expertise for readout. Here, we report an integrated Competitive Volumetric-bar-chart Chip (CV-Chip) to assay multiple drug targets at the point-of-care (POC). To the best of our knowledge, it is the first time that a POC platform has been demonstrated to fully address the above-mentioned limitations. We applied this integrated CV-Chip platform to assay multiple drugs in 38 patient urine and serum samples and validated the on-chip results with an LC-MS/MS method, indicating a clinical sensitivity and specificity of 0.94 and 1.00, respectively. We further demonstrated that the combination of an on-chip blood separator with the CV-Chip enabled the platform to directly assay finger-prick whole blood samples, which has always been recognized as an ideal biospecimen for POC detections. In summary, this integrated CV-Chip is able to serve as a sensitive, accurate, fast, portable, readout visible, and minimally invasive platform for drug abuse assessment.
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影响因子：
5.9
作者：
Li, Guohua;Brady, Joanne E.;Chen, Qixuan
通讯作者：
Chen, Qixuan
影响因子：
7.4
作者：
Espy, Ryan D.;Teunissen, Sebastiaan Frans;Cooks, R. Graham
通讯作者：
Cooks, R. Graham
影响因子：
2.8
作者：
Lee, Yungkang;Lai, Keane K. Y.;Sadrzadeh, S. M. Hossein
通讯作者：
Sadrzadeh, S. M. Hossein
影响因子：
17.1
作者：
Li Y;Xuan J;Song Y;Qi W;He B;Wang P;Qin L
通讯作者：
Qin L
影响因子：
4.2
作者：
Castanheira, Ana P.;Barbosa, Ana I.;Reis, Nuno M.
通讯作者：
Reis, Nuno M.