Using organoids to investigate human endometrial receptivity.
Using organoids to investigate human endometrial receptivity.
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DOI：
10.3389/fendo.2023.1158515
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发表时间：
2023
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
人的子宫内膜只能在每个月经周期的分泌中期接受植入胚泡。这种依赖时间的功能变化需要各种因素复杂的相互作用，主要由雌激素和黄体酮协调。子宫内膜容受性异常被认为是人类植入失败的三分之二原因，因此显著阻碍了试管受精的成功。尽管子宫内膜容受性在着床过程中的重要性是毋庸置疑的，但调控子宫内膜容受性的确切机制仍不明确。这主要是由于缺乏合适的体外模型来概括接受性强的人子宫内膜的体内环境。最近从人类子宫内膜中建立了有机类化合物，具有更好地模拟接受期的前景。子宫内膜类器官显示出长期的可扩张性和保存子宫内膜起源组织的结构和功能特征的能力。这个三维模型在体外保持了对类固醇激素的良好反应，并复制了体内接受性子宫内膜的关键形态特征，包括针脚和假复层上皮。在这里，我们回顾了目前子宫内膜器质性研究的结果，这些研究集中在研究子宫内膜的容受性，并着重于进一步完善和改进这一模型的方法。
The human endometrium is only receptive to an implanting blastocyst in the mid-secretory phase of each menstrual cycle. Such time-dependent alterations in function require intricate interplay of various factors, largely coordinated by estrogen and progesterone. Abnormal endometrial receptivity is thought to contribute to two-thirds of the implantation failure in humans and therefore significantly hindering IVF success. Despite the incontrovertible importance of endometrial receptivity in implantation, the precise mechanisms involved in the regulation of endometrial receptivity remain poorly defined. This is mainly due to a lack of proper in vitro models that recapitulate the in vivo environment of the receptive human endometrium. Organoids were recently established from human endometrium with promising features to better mimic the receptive phase. Endometrial organoids show long-term expandability and the capability to preserve the structural and functional characteristics of the endometrial tissue of origin. This three-dimensional model maintains a good responsiveness to steroid hormones in vitro and replicates key morphological features of the receptive endometrium in vivo, including pinopodes and pseudostratified epithelium. Here, we review the current findings of endometrial organoid studies that have been focused on investigating endometrial receptivity and place an emphasis on methods to further refine and improve this model.
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DOI：
10.3390/medicina54050069
发表时间：
2018-10-10
期刊：
Medicina (Kaunas, Lithuania)
影响因子：
--
作者：
Aunapuu M;Kibur P;Järveots T;Arend A
通讯作者：
Arend A
影响因子：
4.6
作者：
Boretto, Matteo;Cox, Benoit;Vankelecom, Hugo
通讯作者：
Vankelecom, Hugo
影响因子：
4.6
作者：
Griffiths, Meaghan;Van Sinderen, Michelle;Dimitriadis, Evdokia
通讯作者：
Dimitriadis, Evdokia
影响因子：
3.3
作者：
Azkargorta, Mikel;Bregon-Villahoz, Marta;Elortza, Felix
通讯作者：
Elortza, Felix
影响因子：
2.5
作者：
Bhagwat, Sonali R.;Redij, Tejashree;Sachdeva, Geetanjali
通讯作者：
Sachdeva, Geetanjali