Colour opponency is widespread across the mouse subcortical visual system and differentially targets GABAergic and non-GABAergic neurons.
Colour opponency is widespread across the mouse subcortical visual system and differentially targets GABAergic and non-GABAergic neurons.
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DOI：
10.1038/s41598-023-35885-z
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发表时间：
2023-06-08
影响因子：
4.6
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中科院分区：
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颜色视觉在动物行为中扮演着许多重要的角色，但令人惊讶的是，对处理颜色的大脑通路仍然知之甚少，包括最常用的实验室哺乳动物小鼠。事实上，小鼠视网膜组织的特定特征在定义小鼠的色觉机制方面提出了挑战，并导致了这可能基本上依赖于“非经典”视杆细胞的建议。相比之下，使用具有改变的锥光谱敏感性的小鼠的研究，以促进光感受器选择性刺激的应用，已经揭示了在皮层下视觉系统中广泛的锥反射。要确定在何种程度上这样的研究结果是真正反映野生型小鼠的色觉，并促进神经回路映射的颜色处理途径，使用交叉遗传的方法，我们在这里建立和验证刺激选择性操纵本地小鼠S-和M-视锥视蛋白类的兴奋。然后，我们使用这些来确认在小鼠视觉丘脑和顶盖前区广泛出现的圆锥神经元（> 25%的神经元）。我们进一步扩展了这些方法，以绘制关键非图像形成视觉中心（顶盖前和膝状体间小叶/腹侧外侧膝状体; IGL/vLGN）中光遗传学鉴定的GABA能（GAD 2表达）细胞的颜色依赖性的发生。引人注目的是，在整个过程中，我们发现S-ON/M-OFF的特异性在非GABA能细胞中富集，在IGL/VLGN中鉴定的GABA能细胞完全缺乏这种性质。总的来说，因此，我们建立了一个重要的新的方法来研究锥功能的小鼠，确认了一个令人惊讶的广泛的外观锥对手处理在小鼠视觉系统和提供新的见解功能专业化的途径处理这些信号。
Colour vision plays many important roles in animal behaviour but the brain pathways processing colour remain surprisingly poorly understood, including in the most commonly used laboratory mammal, mice. Indeed, particular features of mouse retinal organisation present challenges in defining the mechanisms underlying colour vision in mice and have led to suggestions that this may substantially rely on ‘non-classical’ rod-cone opponency. By contrast, studies using mice with altered cone spectral sensitivity, to facilitate application of photoreceptor-selective stimuli, have revealed widespread cone-opponency across the subcortical visual system. To determine the extent to which such findings are truly reflective of wildtype mouse colour vision, and facilitate neural circuit mapping of colour-processing pathways using intersectional genetic approaches, we here establish and validate stimuli for selectively manipulating excitation of the native mouse S- and M-cone opsin classes. We then use these to confirm the widespread appearance of cone-opponency (> 25% of neurons) across the mouse visual thalamus and pretectum. We further extend these approaches to map the occurrence of colour-opponency across optogenetically identified GABAergic (GAD2-expressing) cells in key non-image forming visual centres (pretectum and intergeniculate leaflet/ventral lateral geniculate; IGL/vLGN). Strikingly, throughout, we find S-ON/M-OFF opponency is specifically enriched in non-GABAergic cells, with identified GABAergic cells in the IGL/VLGN entirely lacking this property. Collectively, therefore, we establish an important new approach for studying cone function in mice, confirming a surprisingly extensive appearance of cone-opponent processing in the mouse visual system and providing new insight into functional specialisation of the pathways processing such signals.
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影响因子：
3.7
作者：
Brown TM;Allen AE;al-Enezi J;Wynne J;Schlangen L;Hommes V;Lucas RJ
通讯作者：
Lucas RJ
影响因子：
9.8
作者：
Brown TM;Gias C;Hatori M;Keding SR;Semo M;Coffey PJ;Gigg J;Piggins HD;Panda S;Lucas RJ
通讯作者：
Lucas RJ
影响因子：
2.9
作者：
Evangelio M;García-Amado M;Clascá F
通讯作者：
Clascá F
影响因子：
16.2
作者：
Chang, Le;Breuninger, Tobias;Euler, Thomas
通讯作者：
Euler, Thomas
影响因子：
1.9
作者：
Ekesten, B;Gouras, P
通讯作者：
Gouras, P