Characteristics of HPV-specific antibody responses induced by infection and vaccination: cross-reactivity, neutralizing activity, avidity and IgG subclasses.
Characteristics of HPV-specific antibody responses induced by infection and vaccination: cross-reactivity, neutralizing activity, avidity and IgG subclasses.
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DOI：
10.1371/journal.pone.0074797
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
van der Klis FR
中科院分区：
文献类型：
--
作者：
Scherpenisse M;Schepp RM;Mollers M;Meijer CJ;Berbers GA;van der Klis FR
为了评估 HPV 特异性 IgG 特征，我们评估了体液抗体反应的多个方面，这将有助于深入了解 HPV 感染和疫苗接种诱导的 HPV 体液免疫反应。使用针对 HPV16、18、31、33、45、52 和 58 的基于 VLP 的多重免疫测定 (MIA)，通过 VLP16 或 18 抑制来评估感染或疫苗接种诱导的 HPV 特异性抗体的交叉反应性。在 HPV 感染和疫苗接种后，使用 VLP-MIA 测定血清中的 HPV16/18 特异性 IgG1-4 亚类和亲和力。在一小部分单血清阳性和多血清阳性天然衍生抗体中测定了中和抗体。来自单阳性血清的天然抗体具有高度基因型特异性，因为同源 VLP 抑制百分比在 78-94% 之间变化。在多阳性血清中，在 α7 和 α9 物种内部以及之间观察到交叉反应抗体。接种疫苗后，交叉反应抗体主要是物种特异性的。疫苗衍生的 HPV 特异性抗体的亲和力比 HPV 感染诱导的抗体的亲和力高 3 倍 (p<0.0001)。发现 IgG1 和 IgG3 是 HPV 感染和疫苗接种后观察到的主要亚类。在测试的小子集中，具有中和能力的单一阳性血清的数量高于多重阳性血清的数量。与来自多阳性血清的抗体相比，来自单阳性样本的天然 HPV 特异性抗体在交叉反应性和中和能力方面表现出不同的特征。接种疫苗后，HPV 抗体亲和力比 HPV 感染诱导的抗体亲和力高约 3 倍。因此，抗体亲和力可能是保护的潜在替代品。
In order to assess HPV-specific IgG characteristics, we evaluated multiple aspects of the humoral antibody response that will provide insight in the HPV humoral immune response induced by HPV infection and vaccination. Cross-reactivity of HPV-specific antibodies induced by infection or vaccination was assessed with VLP16 or 18 inhibition using a VLP-based multiplex immunoassay (MIA) for HPV16, 18, 31, 33, 45, 52 and 58. HPV16/18 specific IgG1-4 subclasses and avidity were determined with the VLP-MIA in sera after HPV infection and after vaccination. Neutralizing antibodies were determined in a small subset of single-seropositive and multi-seropositive naturally derived antibodies. Naturally derived antibodies from single-positive sera were highly genotype-specific as homologue VLP-inhibition percentages varied between 78-94%. In multi-positive sera, cross-reactive antibodies were observed both within and between α7 and α9 species. After vaccination, cross-reactive antibodies were mainly species-specific. Avidity of vaccine-derived HPV-specific antibodies was 3 times higher than that of antibodies induced by HPV infection (p<0.0001). IgG1 and IgG3 were found to be the predominant subclasses observed after HPV infection and vaccination. In the small subset tested, the number of single-positive sera with neutralizing capacity was higher than of multi-positive sera. Naturally derived HPV-specific antibodies from single-positive samples showed different characteristics in terms of cross-reactivity and neutralizing capacity compared with antibodies from multi-positive sera. Post-vaccination, HPV antibody avidity was approximately 3 times higher than antibody avidity induced by HPV infection. Therefore, antibody avidity might be a potential surrogate of protection.
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DOI：
10.4161/hv.7.12.18281
发表时间：
2011-12-01
期刊：
HUMAN VACCINES
影响因子：
--
作者：
Einstein, Mark H.;Baron, Mira;Dubin, Gary
通讯作者：
Dubin, Gary
影响因子：
1.7
作者：
Burk, Robert D.;Chen, Zigui;Van Doorslaer, Koenraad
通讯作者：
Van Doorslaer, Koenraad
影响因子：
6.4
作者：
Anttila, M;Eskola, J;Käyhty, H
通讯作者：
Käyhty, H
影响因子：
2.9
作者：
Heim, Kurt;Hudelist, Gernot;Hoepfl, Reinhard
通讯作者：
Hoepfl, Reinhard
影响因子：
--
作者：
Palmroth, Johanna;Namujju, Proscovia;Lehtinen, Matti
通讯作者：
Lehtinen, Matti