Inbred mouse strains C57BL/6J and DBA/2J vary in sensitivity to a subset of bitter stimuli.
Inbred mouse strains C57BL/6J and DBA/2J vary in sensitivity to a subset of bitter stimuli.
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DOI：
10.1186/1471-2156-6-36
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发表时间：
2005-06-20
期刊：
影响因子：
2.9
通讯作者：
Munger SD
中科院分区：
文献类型：
--
作者：
Boughter JD Jr;Raghow S;Nelson TM;Munger SD
常见的近亲繁殖的小鼠品系具有典型的基因多样性，但人们对这种多样性与特定行为差异的关系仍然知之甚少。为了确定影响味觉行为差异的数量性状基因，利用专门测量味觉线索贡献的分析是至关重要的。除了少数例外，以前对近亲繁殖的小鼠品系的行为味觉敏感性的描述通常是测量消耗量，这可能与摄入后的影响相混淆。在这里，我们使用味觉显著性简短访问程序来测量C57BL/6J （B6）和DBA/2J （D2）小鼠对8种苦味或厌恶刺激的味觉敏感性。B6小鼠比D2小鼠对盐酸奎宁（QHCl）、6-n-丙基硫脲嘧啶（PROP）和MgCl2等苦味刺激更敏感。D2小鼠比B6小鼠对苦刺激棉子糖（RUA）更敏感。这些菌株对环己亚胺（CYX）、苯甲酸地那铵（DB）、KCl或HCl的敏感性没有差异。B6-D2味觉敏感性的差异表明，QHCl、PROP、MgCl2和RUA摄入的差异是基于即时的口感信号，而不是摄食后的影响。CYX的菌株差异的缺失表明，trr型味觉受体的多态性在体外对CYX有不同的敏感性，不太可能在体内对CYX行为反应有不同的贡献。这些研究的结果表明，这些常见的小鼠品系及其相关资源对味觉遗传机制的研究具有实用价值。
Common inbred mouse strains are genotypically diverse, but it is still poorly understood how this diversity relates to specific differences in behavior. To identify quantitative trait genes that influence taste behavior differences, it is critical to utilize assays that exclusively measure the contribution of orosensory cues. With a few exceptions, previous characterizations of behavioral taste sensitivity in inbred mouse strains have generally measured consumption, which can be confounded by post-ingestive effects. Here, we used a taste-salient brief-access procedure to measure taste sensitivity to eight stimuli characterized as bitter or aversive in C57BL/6J (B6) and DBA/2J (D2) mice. B6 mice were more sensitive than D2 mice to a subset of bitter stimuli, including quinine hydrochloride (QHCl), 6-n-propylthiouracil (PROP), and MgCl2. D2 mice were more sensitive than B6 mice to the bitter stimulus raffinose undecaacetate (RUA). These strains did not differ in sensitivity to cycloheximide (CYX), denatonium benzoate (DB), KCl or HCl. B6-D2 taste sensitivity differences indicate that differences in consumption of QHCl, PROP, MgCl2 and RUA are based on immediate orosensory cues, not post-ingestive effects. The absence of a strain difference for CYX suggests that polymorphisms in a T2R-type taste receptor shown to be differentially sensitive to CYX in vitro are unlikely to differentially contribute to the CYX behavioral response in vivo. The results of these studies point to the utility of these common mouse strains and their associated resources for investigation into the genetic mechanisms of taste.
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影响因子：
30.8
作者：
Bufe, B;Hofmann, T;Meyerhof, W
通讯作者：
Meyerhof, W
影响因子：
2.9
作者：
Frank, ME;Blizard, DA
通讯作者：
Blizard, DA
影响因子：
64.5
作者：
Adler, E;Hoon, MA;Zuker, CS
通讯作者：
Zuker, CS
影响因子：
64.5
作者：
Chandrashekar, J;Mueller, KL;Ryba, NJP
通讯作者：
Ryba, NJP
影响因子：
3.5
作者：
Dotson, CD;Spector, AC
通讯作者：
Spector, AC