Low-temperature stress affects reactive oxygen species, osmotic adjustment substances, and antioxidants in rice (Oryza sativa L.) at the reproductive stage.
Low-temperature stress affects reactive oxygen species, osmotic adjustment substances, and antioxidants in rice (Oryza sativa L.) at the reproductive stage.
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DOI：
10.1038/s41598-022-10420-8
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发表时间：
2022-04-13
影响因子：
4.6
通讯作者：
Pan, Guojun
中科院分区：
文献类型：
--
作者：
Guo, Zhenhua;Cai, Lijun;Liu, Chuanxue;Chen, Zhiqiang;Guan, Shiwu;Ma, Wendong;Pan, Guojun
水稻对低温胁迫（LTS）的敏感性，尤其是繁殖期的敏感性，是冷作区水稻产量波动的首要因素。本文分析了水稻生殖阶段低温（LT）生长过程中不同组织中活性氧（ROS）、渗透调节物质和抗氧化剂的变化。结果表明，LTS 增加了 LJ25（LTS 抗性）和 LJ11（LTS 敏感）中脯氨酸 (Pro)、可溶性蛋白 (SP)、谷胱甘肽 (GSH)、超氧化物酶 (SOD) 和抗坏血酸过氧化物酶 (APX) 的水平。 LTS处理下，LJ25的过氧化氢酶（CAT）和过氧化物酶（POD）活性显着增加，而LJ11的过氧化氢酶（POD）活性显着增加，而ROS和丙二醛（MDA）的活性则相反。此外，旗叶和圆锥花序的大部分理化特性均高于叶鞘。 OsCOIN、OsCATC、OsMAP1、OsPOX1和OsAPX的表达模式与Pro及其编码的酶的表型变化相同，证实了理化分析的准确性。因此，LJ25中只有CAT和POD增加较多，这表明它们可能是寒地水稻耐LT育种的关键因素。
The sensitivity of rice to low-temperature stress (LTS), especially at the reproductive stage, is a primary factor of rice yield fluctuation in cold cultivate region. Here, the changes of reactive oxygen species (ROS), osmotic adjustment substances, and antioxidants in different tissues were analyzed during rice growing under low temperatures (LT) at the reproductive stage. Results showed that LTS increases the levels of proline (Pro), soluble protein (SP), glutathione (GSH), superoxidase (SOD), and ascorbate peroxidase (APX) in LJ25 (LTS-resistant) and LJ11 (LTS-sensitive). The activities of catalase (CAT) and peroxidase (POD) were significantly increased in LJ25 but decreased in LJ11 under LTS, while an opposite trend in ROS and malondialdehyde (MDA) was observed in both varieties. Moreover, most physicochemical properties were higher in flag leaves and panicles compared with those in leaf sheaths. The expression patterns of OsCOIN, OsCATC, OsMAP1, OsPOX1, and OsAPX were the same with phenotypic changes in Pro and the enzymes encoded by them, confirming the accuracy of the physicochemical analysis. Therefore, only CAT and POD increased more in LJ25, suggesting they could be the key factors used for LT-tolerant breeding of rice in cold regions.
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影响因子：
3.5
作者：
Guo Z;Cai L;Chen Z;Wang R;Zhang L;Guan S;Zhang S;Ma W;Liu C;Pan G
通讯作者：
Pan G
DOI：
10.1007/978-1-60761-913-0_13
发表时间：
2011-01-01
期刊：
PROTEIN CHROMATOGRAPHY: METHODS AND PROTOCOLS
影响因子：
--
作者：
Campion, Eva M.;Loughran, Sinead T.;Walls, Dermot
通讯作者：
Walls, Dermot
影响因子：
4.6
作者：
Hajihashemi, Shokoofeh;Brestic, Marian;Skalicky, Milan
通讯作者：
Skalicky, Milan
影响因子：
5.5
作者：
Jia, Yan;Liu, Hualong;Zhao, Hongwei
通讯作者：
Zhao, Hongwei
影响因子：
4.3
作者：
Liu, Kaimao;Wang, Lei;Chong, Kang
通讯作者：
Chong, Kang