Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq.
Identification of candidate genes controlling chilling tolerance of rice in the cold region at the booting stage by BSA-Seq and RNA-Seq.
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DOI：
10.1098/rsos.201081
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发表时间：
2020-11
影响因子：
3.5
通讯作者：
Pan G
中科院分区：
文献类型：
--
作者：
Guo Z;Cai L;Chen Z;Wang R;Zhang L;Guan S;Zhang S;Ma W;Liu C;Pan G
水稻对低温很敏感，特别是在孕穗期。水稻耐冷性是一个多基因控制的数量性状位点，利用常规方法对其进行精确鉴定是一项艰巨的任务。本研究对水稻孕穗期耐冷性相关候选基因进行了研究。F2群体由耐冷品种龙粳25和冷敏感品种龙粳11杂交而成。构建了两个混合分离分析池。在第6和第9染色体上的一个0.82 Mb的区域中包含98个注释基因，被认为是与水稻孕穗期耐冷性相关的候选区域。对龙粳25和龙粳11进行转录组学分析，在候选区间上发现了50个差异表达基因。进行DEG的KEGG途径富集分析。9个途径被发现是丰富的，其中包含10个DEG。共有4个基因在龙粳25和龙粳11之间具有不同的表达模式或表达水平。其中4个DEG被认为是潜在的耐冷性候选基因。本研究为水稻耐冷性候选基因的克隆和分子育种提供了基础，为培育耐冷水稻品种奠定了基础。
Rice is sensitive to low temperatures, specifically at the booting stage. Chilling tolerance of rice is a quantitative trait loci that is governed by multiple genes, and thus, its precise identification through the conventional methods is an arduous task. In this study, we investigated the candidate genes related to chilling tolerance at the booting stage of rice. The F2 population was derived from Longjing25 (chilling-tolerant) and Longjing11 (chilling-sensitive) cross. Two bulked segregant analysis pools were constructed. A 0.82 Mb region containing 98 annotated genes on chromosomes 6 and 9 was recognized as the candidate region associated with chilling tolerance of rice at the booting stage. Transcriptomic analysis of Longjing25 and Longjing11 revealed 50 differentially expressed genes (DEGs) on the candidate intervals. KEGG pathway enrichment analysis of DEGs was performed. Nine pathways were found to be enriched, which contained 10 DEGs. A total of four genes had different expression patterns or levels between Longjing25 and Longjing11. Four out of the 10 DEGs were considered as potential candidate genes for chilling tolerance. This study will assist in the cloning of the candidate genes responsible for chilling tolerance and molecular breeding of rice for the development of chilling-tolerant rice varieties.
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影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y
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作者：
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Blamey, FPC
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作者：
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通讯作者：
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影响因子：
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作者：
CHALMERS, KJ;BARUA, UM;POWELL, W
通讯作者：
POWELL, W
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作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
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