Meiotic chromosome dynamics dependent upon the rec8(+), rec10(+) and rec11(+) genes of the fission yeast Schizosaccharomyces pombe.
Meiotic chromosome dynamics dependent upon the rec8(+), rec10(+) and rec11(+) genes of the fission yeast Schizosaccharomyces pombe.
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减数分裂染色体动力学依赖于裂殖酵母裂殖酵母的rec8( )、rec10( ) 和rec11( ) 基因。
DOI：
10.1093/genetics/153.1.57
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发表时间：
1999
期刊：
影响因子：
3.3
通讯作者：
Wahls,WP
中科院分区：
文献类型：
--
作者：
Krawchuk,MD;DeVeaux,LC;Wahls,WP
在减数分裂过程中，同源染色体复制一次、配对、重组，并经历两轮分离，产生单倍体减数分裂产物。裂殖酵母粟酒裂殖酵母的rec8+、rec10+和rec11+基因对于减数分裂重组表现出相似的特异性，并且rec8+是姐妹染色单体凝聚和同源配对所必需的。我们应用细胞学和遗传学方法来识别潜在的遗传相互作用并测量突变体中减数分裂染色体分离的保真度。 rec8+基因相对于rec10+和rec11+具有上位性，但rec10+和rec11+之间没有明确的上位关系。在rec突变体中，所有三个染色体中央区域的相互（交叉）重组受到损害，但端粒附近的重组几乎正常。每个突变体还表现出所有三个染色体的高异常分离率。 rec8 突变主要影响减数分裂 I 分离。值得注意的是，rec10和rec11突变损害了减数分裂I期间的重组，主要影响减数分裂II的分离。我们认为这些基因编码“减数分裂染色单体凝聚力”途径的调节因子或成分，该途径涉及建立、维持和适当释放染色体之间的减数分裂相互作用。姐妹染色单体凝聚力和交叉位置之间的协同相互作用模型表明，交叉和凝聚力如何帮助确保每个减数分裂中染色体的正确分离。
During meiosis homologous chromosomes replicate once, pair, experience recombination, and undergo two rounds of segregation to produce haploid meiotic products. The rec8+, rec10+, and rec11+genes of the fission yeast Schizosaccharomyces pombe exhibit similar specificities for meiotic recombination and rec8+is required for sister chromatid cohesion and homolog pairing. We applied cytological and genetic approaches to identify potential genetic interactions and to gauge the fidelity of meiotic chromosome segregation in the mutants. The rec8+gene was epistatic to rec10+and to rec11+, but there was no clear epistatic relationship between rec10+and rec11+. Reciprocal (crossover) recombination in the central regions of all three chromosomes was compromised in the rec mutants, but recombination near the telomeres was nearly normal. Each of the mutants also exhibited a high rate of aberrant segregation for all three chromosomes. The rec8 mutations affected mainly meiosis I segregation. Remarkably, the rec10 and rec11 mutations, which compromised recombination during meiosis I, affected mainly meiosis II segregation. We propose that these genes encode regulators or components of a “meiotic chromatid cohesion” pathway involved in establishing, maintaining, and appropriately releasing meiotic interactions between chromosomes. A model of synergistic interactions between sister chromatid cohesion and crossover position suggests how crossovers and cohesion help ensure the proper segregation of chromosomes in each of the meiotic divisions.
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影响因子：
3.3
作者：
L. DeVeaux;Nicole A. Hoagland;Gerald R. Smith
通讯作者：
L. DeVeaux;Nicole A. Hoagland;Gerald R. Smith
Tatebayashi, K.、Kato, J.、Ikeda, H.：“分离一种在染色体分离、微管功能、DNA 修复和羟基脲敏感方面异常的粟酒裂殖酵母 rad21ts 突变体：Rad21 可能参与泛素介导
DOI：
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发表时间：
--
期刊：
影响因子：
--
作者：
通讯作者：
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DOI：
10.1083/jcb.140.5.1003
发表时间：
1998-03-09
期刊：
The Journal of cell biology
影响因子：
--
作者：
Moore DP;Page AW;Tang TT;Kerrebrock AW;Orr-Weaver TL
通讯作者：
Orr-Weaver TL
影响因子：
3.5
作者：
Koehler, KE;Hawley, RS;Hassold, T
通讯作者：
Hassold, T
影响因子：
14.9
作者：
BIRKENBIHL, RP;SUBRAMANI, S
通讯作者：
SUBRAMANI, S