Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis.
Comparison of mesenchymal stem cells obtained by suspended culture of synovium from patients with rheumatoid arthritis and osteoarthritis.
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DOI：
10.1186/s12891-018-1998-6
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发表时间：
2018-03-09
影响因子：
2.3
通讯作者：
Sekiya I
中科院分区：
文献类型：
--
作者：
Kohno Y;Mizuno M;Ozeki N;Katano H;Otabe K;Koga H;Matsumoto M;Kaneko H;Takazawa Y;Sekiya I
使用骨关节炎（OA）的“悬浮滑膜培养模型”揭示了来自滑膜的间充质干细胞（MSC）的动员。类风湿性关节炎（RA）的病理不同于OA。我们调查了是否动员间充质干细胞从滑膜也发生在RA，我们比较了从RA和OA的滑膜悬浮培养模型收集的滑膜间充质干细胞的属性。在全膝关节置换术期间，从RA（n = 8）和OA（n = 6）患者的膝关节采集人类滑膜。将滑膜悬浮在含有培养基的瓶中，底部为培养皿。从培养皿中收获细胞并进行分析。RA和OA之间在每克滑膜收获的细胞数方面没有观察到显著差异。然而，从每个供体中收获的细胞数量的变化对于RA大于OA。收获的细胞是多能的，RA和OA之间的软骨颗粒重量没有观察到差异。RA和OA细胞的表面抗原表位与MSCs相似。使用RA的悬浮滑膜培养模型证明了来自滑膜的MSC的动员。RA和OA模型之间收获的细胞数量、软骨形成潜力和表面表位谱相当。
Mobilization of mesenchymal stem cells (MSCs) from the synovium was revealed using a “suspended synovium culture model” of osteoarthritis (OA). The pathology of rheumatoid arthritis (RA) differs from that of OA. We investigated whether mobilization of MSCs from the synovium also occurred in RA, and we compared the properties of synovial MSCs collected from suspended synovium culture models of RA and OA. Human synovium was harvested during total knee arthroplasty from the knee joints of patients with RA (n = 8) and OA (n = 6). The synovium was suspended in a bottle containing culture medium and a culture dish at the bottom. Cells were harvested from the dish and analyzed. No significant difference was observed between RA and OA in the harvested cell numbers per g of synovium. However, the variation in the number of cells harvested from each donor was greater for RA than for OA. The harvested cells were multipotent and no difference was observed in the cartilage pellet weight between RA and OA. The surface epitopes of the cells in RA and OA were similar to those of MSCs. Mobilization of MSCs from the synovium was demonstrated using a suspended synovium culture model for RA. The harvested cell numbers, chondrogenic potentials, and surface epitope profiles were comparable between the RA and OA models.
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影响因子：
4.2
作者：
Matsukura Y;Muneta T;Tsuji K;Koga H;Sekiya I
通讯作者：
Sekiya I
影响因子：
2.8
作者：
Matsukura, Yu;Muneta, Takeshi;Tsuji, Kunikazu;Miyatake, Kazumasa;Yamada, Jun;Abula, Kahaer;Koga, Hideyuki;Tomita, Makoto;Sekiya, Ichiro
通讯作者：
Sekiya, Ichiro
影响因子：
4
作者：
Shirasawa, S;Sekiya, I;Muneta, T
通讯作者：
Muneta, T
影响因子：
2.8
作者：
Zhang, Sheng;Muneta, Takeshi;Sekiya, Ichiro
通讯作者：
Sekiya, Ichiro
影响因子：
--
作者：
Haywood, L;McWilliams, DF;Walsh, DA
通讯作者：
Walsh, DA