A test strip for lead(II) based on gold nanoparticles multi-functionalized by DNAzyme and barcode DNA
A test strip for lead(II) based on gold nanoparticles multi-functionalized by DNAzyme and barcode DNA
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基于DNAzyme和条码DNA多功能化金纳米粒子的铅(II)测试条
DOI：
10.1134/s1061934815030247
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发表时间：
2015-03
影响因子：
1.1
通讯作者：
Sui Wang
中科院分区：
文献类型：
--
作者：
Tingting Hao;Xiaohua Jiang;Zhiyong Guo;Sui Wang
开发了一种简单、快速、灵敏和选择性的测试条，用于检测水样中的铅(II)。该方法基于由 17E-17DS 双链体和条形码 DNA 多功能化的金纳米颗粒，AuNPs@（17E-17DS 双链体和条形码 DNA）。通过17E DNAzyme 与其相应的17DS 裂解底物之间的杂交构建17E 和17DS 的复合物（双链体）。由于 17E DNAzyme 对 Lead(II) 具有高度特异性，因此 Lead(II) 的存在会诱导 17E DNAzyme 特异性裂解 17DS。采用与捕获DNA互补的条形码DNA来减少17E-17DS双链体的使用量，并增加切割的AuNPs@（17E-17DS双链体和条形码DNA）缀合物的捕获概率，从而有效提高检测灵敏度。无需任何仪器和样品预处理，10分钟内即可目测获得铅(II)的检测结果，检出限为20 nM。此外，通过用其他二价金属离子挑战传感器来研究该传感系统的选择性，没有发现明显的干扰。因此，所开发的测试条可能成为快速检测铅（II）的潜在现场筛查工具。
A simple, rapid, sensitive and selective test strip for the detection of lead(II) in water samples was developed. This method is based on gold nanoparticles multi-functionalized by 17E-17DS duplex and barcode DNA, AuNPs@(17E-17DS duplex & barcode DNA). The complex of 17E and 17DS (duplex) was constructed by the hybridization between 17E DNAzyme and its corresponding 17DS cleavage substrate. Because 17E DNAzyme has high specificity for lead(II), the presence of lead(II) induces 17E DNAzyme to specifically cleave 17DS. A barcode DNA complementary to the capture DNA was employed to reduce the amount of 17E-17DS duplex used and increase the capture probability of cleaved AuNPs@(17E-17DS duplex & barcode DNA) conjugates, and thus effectively improve the detection sensitivity. The test results for lead(II) could be visually obtained within 10 min without any instrument and sample pretreatment, with a detection limit 20 nM. In addition, the selectivity of this sensing system was investigated by challenging the sensor with other divalent metal ions, and no significant interference was found. Therefore, the developed test strip could be a potential on-site screening tool for the rapid detection of lead(II).
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基于金属离子触发DNA切割DNAzyme和DNA嵌入剂的Cu2+无标记荧光分子开关
影响因子：
3.3
作者：
Wei, Mingjie;Yi, Yinhui;Li, Haitao;Yao, Shouzhuo
通讯作者：
Yao, Shouzhuo
影响因子：
6.1
作者：
Goluch, Edgar D.;Nam, Jwa-Min;Liu, Chang
通讯作者：
Liu, Chang
影响因子：
13.3
作者：
Oh, BK;Nam, JM;Mirkin, CA
通讯作者：
Mirkin, CA
影响因子：
7.4
作者：
GRABAR, KC;FREEMAN, RG;NATAN, MJ
通讯作者：
NATAN, MJ
DOI：
10.1016/j.chroma.2013.04.060
发表时间：
2013-06
期刊：
Journal of chromatography. A
影响因子：
--
作者：
G. Zachariadis
通讯作者：
G. Zachariadis