Low light CMOS contact imager with an integrated poly-acrylic emission filter for fluorescence detection.
Low light CMOS contact imager with an integrated poly-acrylic emission filter for fluorescence detection.
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DOI：
10.3390/s100505014
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Yadid-Pecht O
中科院分区：
文献类型：
--
作者：
Dattner Y;Yadid-Pecht O
本研究介绍了一种低成本聚丙烯酸 (PAA) 发射滤光片的制造方法，该滤光片与用于荧光检测的低光 CMOS 接触式成像仪集成。该过程涉及使用 PAA 作为排放过滤器的粘合剂。选择聚丙烯酸溶液是因为其光学透明特性、粘合特性、与极性质子溶剂的混溶性以及最重要的是其与生物环境的生物相容性。发射滤光片，也称为吸收滤光片，涉及将吸收样本溶解在极性质子溶剂中，并将其与 PAA 混合，以均匀地粘合吸收样本并硬化滤光片。 PAA 在固体形式下是光学透明的，因此不会影响可见光谱中的光的吸收。可以衍生出吸收样品和极性质子溶剂的许多组合，从而在光谱的不同部分产生不同的滤光片特性。我们报告了一个特定的组合作为我们技术实施的第一个例子。报道的滤光片利用光敏传感器阵列提高的量子效率，在绿色光谱中激发并在红色光谱中发射。通过使用液体的比尔-朗伯定律、荧光团的量子产率和传感器阵列的量子效率计算所需的信噪比来选择滤光片的厚度 (20 μm)。该滤光片的良好特性使其适用于弱光荧光检测。该滤光片与功能齐全的低噪声、低光 CMOS 接触式成像仪集成，并展示了使用荧光聚苯乙烯微球的实验结果。
This study presents the fabrication of a low cost poly-acrylic acid (PAA) based emission filter integrated with a low light CMOS contact imager for fluorescence detection. The process involves the use of PAA as an adhesive for the emission filter. The poly-acrylic solution was chosen due its optical transparent properties, adhesive properties, miscibility with polar protic solvents and most importantly its bio-compatibility with a biological environment. The emission filter, also known as an absorption filter, involves dissolving an absorbing specimen in a polar protic solvent and mixing it with the PAA to uniformly bond the absorbing specimen and harden the filter. The PAA is optically transparent in solid form and therefore does not contribute to the absorbance of light in the visible spectrum. Many combinations of absorbing specimen and polar protic solvents can be derived, yielding different filter characteristics in different parts of the spectrum. We report a specific combination as a first example of implementation of our technology. The filter reported has excitation in the green spectrum and emission in the red spectrum, utilizing the increased quantum efficiency of the photo sensitive sensor array. The thickness of the filter (20 μm) was chosen by calculating the desired SNR using Beer-Lambert’s law for liquids, Quantum Yield of the fluorophore and the Quantum Efficiency of the sensor array. The filters promising characteristics make it suitable for low light fluorescence detection. The filter was integrated with a fully functional low noise, low light CMOS contact imager and experimental results using fluorescence polystyrene micro-spheres are presented.
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