Identification of Dermatophyte species after implementation of the in-house MALDI-TOF MS database.
Identification of Dermatophyte species after implementation of the in-house MALDI-TOF MS database.
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DOI：
10.3390/ijms150916012
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发表时间：
2014-09-11
影响因子：
5.6
通讯作者：
De Conto F
中科院分区：
文献类型：
--
作者：
Calderaro A;Motta F;Montecchini S;Gorrini C;Piccolo G;Piergianni M;Buttrini M;Medici MC;Arcangeletti MC;Chezzi C;De Conto F
尽管基质辅助激光解吸/电离飞行时间质谱仪(MALDI-TOF)已成为临床微生物学研究的有力工具，但迄今为止，很少有研究集中于基于MALDI-TOF MS的皮肤真菌鉴定。在这项研究中，我们用MALDI-TOF MS分析从临床样本中分离的皮肤真菌菌株，以补充我们实验室现有的参考数据库。用MALDI-TOF MS分析了经常规鉴定和分子标准鉴定的24株皮肤真菌(13株参考菌株和11株野外分离菌株)，获得的光谱用于补充现有的数据库，仅限于少数几种。为了验证实施数据库的稳健性，使用MALDI-TOF MS鉴定了实施中使用的临床分离株。实施该数据库后，可以鉴定原始数据库中没有包括的物种，加强了对已经存在的物种的鉴定，并正确地鉴定了以前被归类为苔藓真菌复合体的苔藓真菌复合体中的菌株。通过分析不同种类皮肤真菌的蛋白质图谱获得的树状图反映了它们的分类，此外，在某些情况下，还显示了数据库中已有的光谱和新添加的光谱之间的不同聚类。在这项研究中，MALDI-TOF MS被证明是一种有用的工具，适用于诊断目的的皮肤真菌鉴定。
Despite that matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) has become a powerful tool in the clinical microbiology setting, few studies have till now focused on MALDI-TOF MS-based identification of dermatophytes. In this study, we analyze dermatophytes strains isolated from clinical samples by MALDI-TOF MS to supplement the reference database available in our laboratory. Twenty four dermatophytes (13 reference strains and 11 field isolated strains), identified by both conventional and molecular standard procedures, were analyzed by MALDI-TOF MS, and the spectra obtained were used to supplement the available database, limited to a few species. To verify the robustness of the implemented database, 64 clinical isolates other than those used for the implementation were identified by MALDI-TOF MS. The implementation allowed the identification of the species not included in the original database, reinforced the identification of the species already present and correctly identified those within the Trichophyton mentagrophytes complex previously classified as Trichophyton. tonsurans by MALDI-TOF MS. The dendrogram obtained by analyzing the proteic profiles of the different species of dermatophytes reflected their taxonomy, showing moreover, in some cases, a different clusterization between the spectra already present in the database and those newly added. In this study, MALDI-TOF MS proved to be a useful tool suitable for the identification of dermatophytes for diagnostic purpose.
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影响因子：
2.9
作者：
L'Ollivier, Coralie;Cassagne, Carole;Ranque, Stephane
通讯作者：
Ranque, Stephane
DOI：
10.1111/j.1610-0387.2007.06180.x
发表时间：
2007-03-01
期刊：
Journal der Deutschen Dermatologischen Gesellschaft = Journal of the German Society of Dermatology : JDDG
影响因子：
--
作者：
Nenoff, Pietro;Herrmann, Jurgen;Graser, Yvonne
通讯作者：
Graser, Yvonne
影响因子：
14.2
作者：
De Carolis, E.;Posteraro, B.;Fadda, G.
通讯作者：
Fadda, G.
影响因子：
3.3
作者：
Calderaro, Adriana;Piccolo, Giovanna;Chezzi, Carlo
通讯作者：
Chezzi, Carlo
影响因子：
9.4
作者：
Theel, Elitza S.;Hall, Leslie;Wengenack, Nancy L.
通讯作者：
Wengenack, Nancy L.