The use of gadolinium-carbon nanostructures to magnetically enhance stem cell retention for cellular cardiomyoplasty.
The use of gadolinium-carbon nanostructures to magnetically enhance stem cell retention for cellular cardiomyoplasty.
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DOI：
10.1016/j.biomaterials.2013.10.013
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发表时间：
2014-01
期刊：
影响因子：
14
通讯作者：
Wilson, Lon J.
中科院分区：
文献类型：
--
作者：
Tran, Lesa A.;Hernandez-Rivera, Mayra;Berlin, Ari N.;Zheng, Yi;Sampaio, Luiz;Bove, Christina;Cabreira-Hansen, Maria da Graca;Willerson, James T.;Perin, Emerson C.;Wilson, Lon J.
关键词：
在这项工作中，评估了在细胞心肌成形术期间使用带有外部磁场的纳米管（GNT）来改善移植的成人间充质干细胞（MSC）保留的有效性。作为一种高性能的T1加权磁共振成像（MRI）细胞跟踪标签，GNT是负载钆的碳纳米管胶囊，使骨髓间充质干细胞的磁性时，内化。用超顺磁性GNT或胶体抗磁性镥（Lu）内部标记MSC。体外细胞滚动试验和离体心脏灌注实验定性地证明了GNT标记的MSC的磁辅助保留增加。随后在缝合到雌性幼年猪（n = 21）左心室上的1.3 T NdFeB环形磁体周围进行体内心外膜细胞注射。改变细胞剂量、磁体暴露时间和终点，以评价所提出的疗法的安全性和有效性。通过元素分析（Gd或Lu）对收集的组织中保留的细胞进行定量显示，外部磁体帮助保留的GNT标记的MSC比Lu标记的细胞多近三倍。缝合磁体耐受长达168小时;然而，48小时后观察到对磁体的炎症反应。这些概念验证研究支持使用GNT作为磁性纳米颗粒促进剂在细胞心肌成形术期间改善细胞保留的可行性和价值。
In this work, the effectiveness of using Gadonanotubes (GNTs) with an external magnetic field to improve retention of transplanted adult mesenchymal stem cells (MSCs) during cellular cardiomyoplasty was evaluated. As a high-performance T1-weighted magnetic resonance imaging (MRI) cell tracking label, the GNTs are gadolinium-loaded carbon nanotube capsules that render MSCs magnetic when internalized. MSCs were internally labeled with either superparamagnetic GNTs or colloidal diamagnetic lutetium (Lu). In vitro cell rolling assays and ex vivo cardiac perfusion experiments qualitatively demonstrated increased magnetic-assisted retention of GNT-labeled MSCs. Subsequent in vivo epicardial cell injections were performed around a 1.3 T NdFeB ring magnet sutured onto the left ventricle of female juvenile pigs (n = 21). Cell dosage, magnet exposure time, and endpoints were varied to evaluate the safety and efficacy of the proposed therapy. Quantification of retained cells in collected tissues by elemental analysis (Gd or Lu) showed that the external magnet helped retain nearly three times more GNT-labeled MSCs than Lu-labeled cells. The sutured magnet was tolerated for up to 168 hours; however, an inflammatory response to the magnet was noted after 48 hours. These proof-of-concept studies support the feasibility and value of using GNTs as a magnetic nanoparticle facilitator to improve cell retention during cellular cardiomyoplasty.
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作者：
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发表时间：
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影响因子：
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