A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish
A landscape of differentiated biological processes involved in the initiation of sex differentiation in zebrafish
复制标题
参与斑马鱼性别分化启动的分化生物过程景观
DOI：
10.1016/j.watbs.2022.100059
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发表时间：
2022-04
期刊：
影响因子：
--
通讯作者：
Yonghua Sun
中科院分区：
文献类型：
--
作者：
Ding Ye;Yi-Xuan Tu;Houpeng Wang;Mudan He;Yaqing Wang;Zhengfang Chen;Zhen-Xia Chen;Yonghua Sun
斑马鱼（Danio rerio）已被用作研究性腺发育和配子发生的有前途的动物模型。尽管之前的研究已经确定了参与斑马鱼性腺分化的关键分子，但仍然缺乏对该过程所涉及的生物过程的全面了解。在这里，我们在受精后 25 天 (dpf) 和 30 天后分离了完整的斑马鱼分化性腺，并对倾向于发育成卵巢或睾丸的幼鱼性腺进行了 RNA-seq 分析。我们的研究表明，25 dpf 和 30 dpf 时的幼年卵巢和睾丸在生物过程水平上是不同的。在卵巢分化过程中，与能量和母体物质产生、RNA降解和DNA修复等代谢活动相关的生物过程被丰富化。睾丸分化过程中，与细胞增殖、分化、形态发生相关的生物学过程丰富，共有15条信号通路。值得注意的是，我们发现免疫相关过程广泛参与睾丸发育的调节。总的来说，这项研究提供了差异化生物过程的图景，并为斑马鱼性别分化的启动提供了新的见解。 • 使用 25 dpf 时种系标记的幼虫斑马鱼分离完整的卵巢和睾丸以进行 RNA 测序分析。 • 幼年卵巢和幼年睾丸在生物过程和信号通路水平上有所不同。 • 免疫相关过程广泛参与幼年睾丸分化的调节。
Zebrafish ( Danio rerio ) has been used as a promising animal model to study gonadal development and gametogenesis. Although previous studies have identified critical molecules participating in zebrafish gonad differentiation, a landscape view of the biological processes involved in this process is still lacking. Here we isolated intact zebrafish differentiating gonads, at 25 days post-fertilization (dpf) and 30 dpf and conducted RNA-seq analyses on the juvenile gonads that tended to develop into ovaries or testes. Our study demonstrates that the juvenile ovary and testis at 25 dpf and 30 dpf are different at the biological process level. During ovary differentiation, the biological processes related to metabolic activities in the production of energy and maternal substances, RNA degradation, and DNA repair were enriched. During testis differentiation, the biological processes related to cell proliferation, differentiation, and morphogenesis were enriched, with a total of 15 signaling pathways. Notably, we reveal that the immune-related processes are extensively involved in the regulation of testis development. Overall, this study provides a landscape of differentiated biological processes and novel insights into the initiation of sex differentiation in zebrafish. •Germline-labeled larval zebrafish at 25 dpf was used to isolate intact ovaries and testes for RNA-seq analysis. • The juvenile ovary and juvenile testis are different at the level of Biological Process and Signaling Pathway. • Immune-related processes are extensively involved in the regulation of juvenile testis differentiation.
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影响因子：
18.9
作者：
Yongge Sun
通讯作者：
Yongge Sun
DOI：
10.1007/978-1-60761-869-0_29
发表时间：
2011-01-01
期刊：
SUPPRESSION AND REGULATION OF IMMUNE RESPONSES: METHODS AND PROTOCOLS
影响因子：
--
作者：
Fijak, Monika;Bhushan, Sudhanshu;Meinhardt, Andreas
通讯作者：
Meinhardt, Andreas
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1002/dvdy.22113
发表时间：
2009-12
期刊：
Developmental dynamics : an official publication of the American Association of Anatomists
影响因子：
--
作者：
Parichy DM;Elizondo MR;Mills MG;Gordon TN;Engeszer RE
通讯作者：
Engeszer RE
影响因子：
1.5
作者：
SELMAN, K;WALLACE, RA;QI, XP
通讯作者：
QI, XP