Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats.
Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats.
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枸杞多糖预防东莨菪碱治疗大鼠的记忆和神经发生损伤
DOI：
10.1371/journal.pone.0088076
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Chen W;Cheng X;Chen J;Yi X;Nie D;Sun X;Qin J;Tian M;Jin G;Zhang X
枸杞在许多国家被用作食品添加剂和药草，而枸杞多糖（LBP）是一种主要的细胞成分，据报道具有广泛的有益作用，包括神经保护、抗衰老和抗癌特性以及免疫调节。 LBP 对神经元功能、神经发生以及药物引起的学习和记忆缺陷的影响尚未得到评估。我们报告了 LBP 对东莨菪碱 (SCO) 治疗大鼠的学习记忆和神经发生的治疗作用。通过胃灌注给予 LBP 2 周，然后再进行皮下 SCO 治疗 4 周。正如预期的那样，SCO 损害了新物体和物体位置识别任务以及莫里斯水迷宫的表现。然而，接受 SCO 和 LBP 双重处理的大鼠在识别任务中花费更多时间探索新物体或位置，并且在水迷宫中的逃避潜伏期显着缩短。 SCO 给药导致齿状回中 Ki67 或 DCX 免疫反应细胞的减少以及新神经元树突发育的损害； LBP 阻止了 SCO 诱导的细胞增殖和神经母细胞分化的减少。 LBP 还可以保护 DCX 免疫反应性神经元中 SCO 诱导的神经元过程损失。生化研究表明，枸杞多糖可降低 SCO 诱导的海马氧化应激，并逆转 SCO 处理后增加的 Bax/Bcl-2 比值。然而，SCO 诱导的 BDNF 减少和 AChE 增加显示对 LBP 给药没有反应。这些结果表明 LBP 可以预防 SCO 引起的认知和记忆缺陷以及细胞增殖和神经母细胞分化的减少。氧化应激和细胞凋亡的抑制可能与 LBP 的上述作用有关，LBP 可能是恢复记忆功能和神经发生的有希望的候选者。
Lycium barbarum is used both as a food additive and as a medicinal herb in many countries, and L. barbarum polysaccharides (LBPs), a major cell component, are reported to have a wide range of beneficial effects including neuroprotection, anti-aging and anticancer properties, and immune modulation. The effects of LBPs on neuronal function, neurogenesis, and drug-induced learning and memory deficits have not been assessed. We report the therapeutic effects of LBPs on learning and memory and neurogenesis in scopolamine (SCO)-treated rats. LBPs were administered via gastric perfusion for 2 weeks before the onset of subcutaneous SCO treatment for a further 4 weeks. As expected, SCO impaired performance in novel object and object location recognition tasks, and Morris water maze. However, dual SCO- and LBP-treated rats spent significantly more time exploring the novel object or location in the recognition tasks and had significant shorter escape latency in the water maze. SCO administration led to a decrease in Ki67- or DCX-immunoreactive cells in the dentate gyrus and damage of dendritic development of the new neurons; LBP prevented these SCO-induced reductions in cell proliferation and neuroblast differentiation. LBP also protected SCO-induced loss of neuronal processes in DCX-immunoreactive neurons. Biochemical investigation indicated that LBP decreased the SCO-induced oxidative stress in hippocampus and reversed the ratio Bax/Bcl-2 that exhibited increase after SCO treatment. However, decrease of BDNF and increase of AChE induced by SCO showed no response to LBP administration. These results suggest that LBPs can prevent SCO-induced cognitive and memory deficits and reductions in cell proliferation and neuroblast differentiation. Suppression of oxidative stress and apoptosis may be involved in the above effects of LBPs that may be a promising candidate to restore memory functions and neurogenesis.
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影响因子：
3.7
作者：
Konar A;Shah N;Singh R;Saxena N;Kaul SC;Wadhwa R;Thakur MK
通讯作者：
Thakur MK
影响因子：
5
作者：
Jeong, Eun Ju;Lee, Ki Yong;Kim, Young Choong
通讯作者：
Kim, Young Choong
影响因子：
3.4
作者：
Couillard-Despres, S;Winner, B;Aigner, L
通讯作者：
Aigner, L
影响因子：
1.2
作者：
Jahanshahi, M.;Nickmahzar, E. G.;Babakordi, F.
通讯作者：
Babakordi, F.
DOI：
10.1002/0470870834.ch14
发表时间：
2000-01-01
期刊：
NEURAL TRANSPLANTATION IN NEURODEGENERATIVE DISEASE: CURRENT STATUS AND NEW DIRECTIONS
影响因子：
--
作者：
Kempermann, G;Gage, FH
通讯作者：
Gage, FH