Albino seedling lethality 4; Chloroplast 30S Ribosomal Protein S1 is Required for Chloroplast Ribosome Biogenesis and Early Chloroplast Development in Rice.
Albino seedling lethality 4; Chloroplast 30S Ribosomal Protein S1 is Required for Chloroplast Ribosome Biogenesis and Early Chloroplast Development in Rice.
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白化苗致死率4；
DOI：
10.1186/s12284-021-00491-y
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发表时间：
2021-05-27
期刊：
影响因子：
--
通讯作者：
Li Z
中科院分区：
文献类型：
--
作者：
Zhou K;Zhang C;Xia J;Yun P;Wang Y;Ma T;Li Z
核糖体负责叶绿体中叶绿体编码蛋白的转录和翻译，对叶绿体的发育和植物生长至关重要。尽管已鉴定出叶绿体中的大部分核糖体蛋白，但调控叶绿体生物发生的分子机制仍有待研究。结果我们鉴定到白化苗变白苗致死性4(As14)是由针对叶绿体的30S核糖体蛋白S1的干扰引起的。该突变体在叶绿体早期发育和叶绿素(Chl)生物合成方面存在缺陷。通过互补试验证实ASL4等位基因2855个碱基缺失是导致突变表型的原因。表达分析表明，在叶片发育早期，ASL4等位基因在叶片4节和新展开的叶片中高表达。在黑暗之后暴露在光线下，表达水平会增加。与野生型相比，突变体组织中参与叶绿体生物发生的一些基因表达上调，另一些基因表达下调。叶绿体编码的叶绿体RNA聚合酶(PEP)依赖的光合作用基因和核编码的噬菌体RNA聚合酶(NEP)依赖的看家基因分别下调和上调，表明该突变体的叶绿体转录受损。转录组和蛋白质印迹分析表明，该突变体中大多数叶绿体编码基因和蛋白质的水平都降低了。叶绿体rRNAs和核糖体蛋白含量的降低表明这14个突变体的叶绿体核糖体生物发生受到损害。结论水稻ASL4编码针对叶绿体的30S核糖体蛋白S1，ASL4是叶绿体核糖体生物发生和叶绿体早期发育所必需的。这些数据将有助于进一步阐明叶绿体生物发生的分子机制。
BackgroundRibosomes responsible for transcription and translation of plastid-encoded proteins in chloroplasts are essential for chloroplast development and plant growth. Although most ribosomal proteins in plastids have been identified, the molecular mechanisms regulating chloroplast biogenesis remain to be investigated.ResultsHere, we identified albinic seedling mutantalbino seedling lethality 4(asl4) caused by disruption of 30S ribosomal protein S1 that is targeted to the chloroplast. The mutant was defective in early chloroplast development and chlorophyll (Chl) biosynthesis. A 2855-bp deletion in theASL4allele was verified as responsible for the mutant phenotype by complementation tests. Expression analysis revealed that theASL4allele was highly expressed in leaf 4 sections and newly expanded leaves during early leaf development. Expression levels were increased by exposure to light following darkness. Some genes involved in chloroplast biogenesis were up-regulated and others down-regulated inasl4mutant tissues compared to wild type. Plastid-encoded plastid RNA polymerase (PEP)-dependent photosynthesis genes and nuclear-encoded phage-type RNA polymerase (NEP)-dependent housekeeping genes were separately down-regulated and up-regulated, suggesting that plastid transcription was impaired in the mutant. Transcriptome and western blot analyses showed that levels of most plastid-encoded genes and proteins were reduced in the mutant. The decreased contents of chloroplast rRNAs and ribosomal proteins indicated that chloroplast ribosome biogenesis was impaired in theasl4mutant.ConclusionsRiceASL4encodes 30S ribosomal protein S1, which is targeted to the chloroplast.ASL4is essential for chloroplast ribosome biogenesis and early chloroplast development. These data will facilitate efforts to further elucidate the molecular mechanism of chloroplast biogenesis.
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影响因子：
6.9
作者：
Liu, Xi;Lan, Jie;Wan, Jianmin
通讯作者：
Wan, Jianmin
DOI：
10.1007/bf02464880
发表时间：
1989-06-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
HIRATSUKA, J;SHIMADA, H;SUGIURA, M
通讯作者：
SUGIURA, M
影响因子：
1.6
作者：
Kusumi, Kensuke;Chono, Yoko;Iba, Koh
通讯作者：
Iba, Koh
影响因子：
7.2
作者：
Pesaresi, P;Varotto, C;Leister, D
通讯作者：
Leister, D
影响因子：
7.2
作者：
De Santis-Maciossek, G;Kofer, W;Herrmann, RG
通讯作者：
Herrmann, RG