Chloroplasts play a central role in facilitating MAMP-triggered immunity, pathogen suppression of immunity and crosstalk with abiotic stress.
Chloroplasts play a central role in facilitating MAMP-triggered immunity, pathogen suppression of immunity and crosstalk with abiotic stress.
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DOI：
10.1111/pce.14408
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发表时间：
2022-10
影响因子：
7.3
通讯作者：
Grant, Murray
中科院分区：
文献类型：
--
作者：
Breen, Susan;Hussain, Rana;Breeze, Emily;Brown, Hannah;Alzwiy, Ibrahim;Abdelsayed, Sara;Gaikwad, Trupti;Grant, Murray
微生物相关分子模式（MAMP）触发免疫（MTI）研究传统上集中于源自激活的膜定位模式识别受体（PRR）的信号转导途径，最终导致核转录和翻译后修饰。最近，叶绿体已成为关键的免疫信号中心，在整合环境信号方面发挥着核心作用。值得注意的是，MAMP 识别会诱导叶绿体活性氧 (cROS) 被病原体效应子抑制，这也会改变叶绿体合成的防御激素前体、茉莉酸、水杨酸 (SA) 和脱落酸的平衡。这项研究的重点是明确表征的 PRR 和辅助受体如何调节叶绿体生理学，检查不同的信号通路是否汇聚以类似地调节叶绿体功能。用 MAMP 和 D（损伤）AMP 肽对受体突变植物进行预处理，通常可以防止叶绿素荧光的效应子调节，并防止丁香假单胞菌效应子介导的 cROS 猝灭和最大暗适应量子效率的抑制（可变/最大荧光的比率 [F v/F m]）。与对照植物相比，MTI 辅助受体双突变体 bak1-5/bkk1-1 在感染期间表现出 F v/F m 显着降低，强调了 MTI 介导的信号在叶绿体免疫中的重要性。进一步探讨叶绿体在免疫中的作用，我们意外地发现，即使光强度的适度变化也可以解开植物免疫信号。
Microbe‐associated molecular pattern (MAMP)‐triggered immunity (MTI) research has traditionally centred around signal transduction pathways originating from activated membrane‐localized pattern recognition receptors (PRRs), culminating in nuclear transcription and posttranslational modifications. More recently, chloroplasts have emerged as key immune signalling hubs, playing a central role in integrating environmental signals. Notably, MAMP recognition induces chloroplastic reactive oxygen species (cROS) that is suppressed by pathogen effectors, which also modify the balance of chloroplast‐synthesized precursors of the defence hormones, jasmonic acid, salicylic acid (SA) and abscisic acid. This study focuses on how well‐characterized PRRs and coreceptors modulate chloroplast physiology, examining whether diverse signalling pathways converge to similarly modulate chloroplast function. Pretreatment of receptor mutant plants with MAMP and D(Damage)AMP peptides usually protect against effector modulation of chlorophyll fluorescence and prevent Pseudomonas syringae effector‐mediated quenching of cROS and suppression of maximum dark‐adapted quantum efficiency (the ratio of variable/maximum fluorescence [F v/F m]). The MTI coreceptor double mutant, bak1‐5/bkk1‐1, exhibits a remarkable decrease in F v/F m compared to control plants during infection, underlining the importance of MTI‐mediated signalling in chloroplast immunity. Further probing the role of the chloroplast in immunity, we unexpectedly found that even moderate changes in light intensity can uncouple plant immune signalling.
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影响因子：
--
作者：
Gould KS
通讯作者：
Gould KS
DOI：
10.3390/plants11040552
发表时间：
2022-02-19
期刊：
Plants (Basel, Switzerland)
影响因子：
--
作者：
Breeze E;Mullineaux PM
通讯作者：
Mullineaux PM
影响因子：
16.6
作者：
Exposito-Rodriguez M;Laissue PP;Yvon-Durocher G;Smirnoff N;Mullineaux PM
通讯作者：
Mullineaux PM
影响因子：
9.4
作者：
Littlejohn, George R.;Gouveia, Joao D.;Love, John
通讯作者：
Love, John
影响因子：
11.4
作者：
de Torres-Zabala, Marta;Truman, William;Grant, Murray
通讯作者：
Grant, Murray