Expression of transcription factors and crystallin proteins during rat lens regeneration
Expression of transcription factors and crystallin proteins during rat lens regeneration
复制标题
大鼠晶状体再生过程中转录因子和晶体蛋白的表达
作者：
目的建立大鼠晶状体再生模型，检测转录因子和晶体蛋白基因的表达。方法采用Sprague-Dawley大鼠囊外晶状体摘除术（ECLE）。在ECLE后的不同时间点进行裂隙灯检查和组织学分析。采用Real-time PCR和/或免疫荧光检测晶状体转录因子配对盒6 （Pax6）、prospero homeobox 1 （Prox1）和forkhead box E3 （Foxe3）以及α-、β-和γ-晶体蛋白（Cryaa、Cryab、Crybb1、Crybb2、Cryba2和Crygd）的表达。结果晶状体上皮细胞（Lens epithelial cells, LECs）在晶状体上皮细胞（ECLE）术后立即在前囊下留下。所有大鼠在ECLE后3天外周囊袋内均出现晶状体纤维分化。手术后1个月，所有囊袋内充满新的半透明透镜状结构，显示出已建立的赤道和分化良好的弓区。ECLE后晶状体转录因子和α-、β-、γ-晶体蛋白mrna表达量增加。Pax6在lec和新生晶状体纤维细胞中均有表达，Prox1在lec和正在分化的晶状体纤维细胞中均有表达，Foxe3仅限于lec。结论晶状体再生过程中晶状体纤维的分化过程与胚胎发育过程相似，上皮细胞沿前后囊增生，后囊上皮细胞伸长，上皮细胞分化为晶状体纤维。再生晶状体含有与正常晶状体相似的蛋白质和转录因子。在晶状体发育过程中所见的诱导相互作用对于晶状体再生不是必需的。
Purpose To establish a model of lens regeneration in rats and to detect the expression of transcription factor and crystallin genes. Methods An extracapsular lens extraction (ECLE) was performed in Sprague-Dawley rats. Examinations with slit-lamp and histological analysis were performed at various time points after ECLE. Real-time PCR and/or immunofluorescence were performed to detect the expression of the lens transcription factors paired box 6 (Pax6), prospero homeobox 1 (Prox1), and forkhead box E3 (Foxe3) and α-, β-, and γ-crystallin (Cryaa, Cryab, Crybb1, Crybb2, Cryba2, and Crygd, respectively). Results Lens epithelial cells (LECs) were left behind under the anterior capsule immediately after ECLE. Lens fiber differentiation had occurred in the peripheral capsular bag in all rats 3 days after ECLE. One month after surgery, all capsular bags were filled with new semitransparent lenticular structures displaying an established equator with well differentiated bow regions. The mRNA-expression quantity of lens transcription factors and α-, β-, and γ- crystallin increased after ECLE. Pax6 was expressed in both LECs and the newly regenerated lens fiber cells, Prox1 was expressed both in LECs and differentiating lens fiber cells, and Foxe3 was confined to LECs. Conclusions Lens fiber differentiation during regeneration follows a process similar to embryological development, with proliferation of epithelial cells along the anterior and posterior capsule, elongation of the posterior epithelial cells, and differentiation of epithelial cells into lens fibers. The regenerated lens contains proteins and transcription factors similar to those found in normal lenses. Inductive interactions seen during lens development are not necessary for lens regeneration.
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影响因子：
4.4
作者：
Lois, N;Dawson, R;Forrester, JV
通讯作者：
Forrester, JV
影响因子：
3.4
作者：
P. Tsonis;E. Fuentes
通讯作者：
P. Tsonis;E. Fuentes
影响因子：
4.4
作者：
K. Del Rio-Tsonis;S. Tomarev;P. Tsonis
通讯作者：
K. Del Rio-Tsonis;S. Tomarev;P. Tsonis
DOI：
10.1073/pnas.0601949103
发表时间：
2006-10-03
影响因子：
11.1
作者：
Madhavan, Mayur;Haynes, Tracy L.;Del Rio-Tsonis, Katia
通讯作者：
Del Rio-Tsonis, Katia
影响因子：
2.2
作者：
Reed, NA;Oh, DJ;Duncan, MK
通讯作者：
Duncan, MK