Visible, safe and certain endotracheal intubation using endoscope system and inhalation anesthesia for rats.
Visible, safe and certain endotracheal intubation using endoscope system and inhalation anesthesia for rats.
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DOI：
10.1292/jvms.14-0146
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发表时间：
2014-10
期刊：
影响因子：
--
通讯作者：
Kasai N
中科院分区：
文献类型：
--
作者：
Konno K;Shiotani Y;Itano N;Ogawa T;Hatakeyama M;Shioya K;Kasai N
麻醉对实验动物的影响很大，对实验数据的影响也很大。在器官移植、再生医学、影像等研究领域，大鼠的使用数量仅次于小鼠。因此，适当和有效的麻醉，包括气管插管和吸入麻醉的方案是至关重要的。因此，我们在本研究中评估了这些方法。12只Wistar大鼠腹腔注射M/M/B: 0.3/4/5，由美托咪定、咪达唑仑和布托啡诺组成，剂量分别为0.3 mg/kg + 4.0 mg/kg + 5.0 mg/kg体重/只。然后将气管内插管插入气管。插管后，用异氟醚将大鼠连接到吸入麻醉回路，并测量生命体征至连接后30min。所有插管均在1 min内顺利完成，生命体征正常稳定。气管、肺组织病理检查未见损伤。可见气管插管方法简单、可靠、安全，有利于大鼠的健康。
Anesthesia strongly influences laboratory animals, and it can also greatly affect the experimental data. Rats rank only second to mice in the number used in research fields, such as organ transplantation, regenerative medicine and imaging. Therefore, appropriate and effective anesthesia, including the protocol of the endotracheal intubation and inhalation anesthesia, is crucial. Hence, we evaluated these methods in this study. Twelve Wistar rats were intraperitoneally injected with M/M/B: 0.3/4/5, comprising of medetomidine, midazolam and butorphanol at a dose of 0.3 mg/kg + 4.0 mg/kg + 5.0 mg/kg body weight/rat, respectively. An endotracheal tube was then intubated into the trachea. After intubation, the rats were connected to the inhalation anesthesia circuit using isoflurane, and vital signs were measured until 30 min after connection. All intubations were successfully finished within 1 min, and the values of the vital signs were normal and stable. In addition, histopathological observation of the trachea and lungs showed no trauma. These results suggest that this visible endotracheal intubation method is simple, reliable, safe and favorable with regard to the rats’ welfare.
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