Oxygen radical absorbance capacity (ORAC) of cyclodextrin-solubilized flavonoids, resveratrol and astaxanthin as measured with the ORAC-EPR method.
Oxygen radical absorbance capacity (ORAC) of cyclodextrin-solubilized flavonoids, resveratrol and astaxanthin as measured with the ORAC-EPR method.
复制标题
用ORAC-EPR方法测量的环糊精，白藜芦醇和astaxanthin的氧自由基吸收能力（ORAC）。
DOI：
10.3164/jcbn.11-21
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发表时间：
2012-03
影响因子：
2.4
通讯作者：
Kotake Y
中科院分区：
文献类型：
--
作者：
Sueishi Y;Ishikawa M;Yoshioka D;Endoh N;Oowada S;Shimmei M;Fujii H;Kotake Y
最近，我们提出了一种氧自由基吸收能力的方法，直接量化的抗氧化剂对自由基的清除能力，并评价水溶性抗氧化剂化合物的自由基清除能力。在这项研究中，我们确定了亲脂性的抗氧化剂的自由基清除能力，这是由环糊精在水中增溶。常用的基于荧光的方法测量抗氧化剂对荧光探针的保护能力，而我们使用电子顺磁共振自旋捕获技术直接定量自由基水平。此外，基于自旋捕获的方法采用偶氮引发剂的受控紫外光分解产生自由基，而在基于荧光的方法中，利用偶氮引发剂的热分解。我们使用甲基化β-环糊精作为增溶剂，测定了七种众所周知的亲脂性抗氧化剂（五种黄酮类化合物、白藜芦醇和虾青素）的自由基清除能力。结果表明，基于自旋捕获的值和基于荧光的值之间的一致性仅部分是公平的，这是由于先前基于荧光的数据中的较大变化。以trolox当量单位计，典型的自由基清除能力为：儿茶素0.96;表儿茶素0.94;表没食子儿茶素没食子酸酯1.3;山奈酚0.37;杨梅素3.2;白藜芦醇0.64;和虾青素0.28，表明杨梅素在测试化合物中具有最高的抗氧化能力。我们梳理了两种方法之间出现偏差的可能原因。
Recently, we proposed an oxygen radical absorbance capacity method that directly quantifies the antioxidant’s scavenging capacity against free radicals and evaluated the radical scavenging abilities for water soluble antioxidant compounds. In this study, we determined the radical scavenging abilities of lipophilic antioxidants which were solubilized by cyclodextrin in water. Commonly employed fluorescence-based method measures the antioxidant’s protection capability for the fluorescent probe, while we directly quantify free-radical level using electron paramagnetic resonance spin trapping technique. In addition, the spin trapping-based method adopted controlled UV-photolysis of azo-initiator for free radical generation, but in fluorescence-based method, thermal decomposition of azo-initiator was utilized. We determined the radical scavenging abilities of seven well-known lipophilic antioxidants (five flavonoids, resveratrol and astaxanthin), using methylated β-cyclodextrin as a solubilizer. The results indicated that the agreement between spin trapping-based and fluorescence-based values was only fair partly because of a large variation in the previous fluorescence-based data. Typical radical scavenging abilities in trolox equivalent unit are: catechin 0.96; epicatechin 0.94; epigallocatechin gallate 1.3; kaempferol 0.37; myricetin 3.2; resveratrol 0.64; and astaxanthin 0.28, indicating that myricetin possesses the highest antioxidant capacity among the compounds tested. We sorted out the possible causes of the deviation between the two methods.
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影响因子：
2.4
作者：
Endo, Nobuyuki;Oowada, Shigeru;Kotake, Yashige
通讯作者：
Kotake, Yashige
影响因子：
3.3
作者：
Kamibayashi, Masato;Oowada, Shigeru;Kotake, Yashige
通讯作者：
Kotake, Yashige
DOI：
10.1039/p29960000747
发表时间：
1996-04-01
期刊：
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 2
影响因子：
--
作者：
Krainev, AG;Bigelow, DJ
通讯作者：
Bigelow, DJ
影响因子：
6.1
作者：
Huang, DJ;Ou, BX;Prior, RL
通讯作者：
Prior, RL
影响因子：
2.9
作者：
DELANGE, RJ;GLAZER, AN
通讯作者：
GLAZER, AN