The role of muscle cells in regulating cartilage matrix production.
The role of muscle cells in regulating cartilage matrix production.
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DOI：
10.1002/jor.21014
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发表时间：
2010-04
期刊：
影响因子：
--
通讯作者：
Zeng L
中科院分区：
文献类型：
--
作者：
Cairns DM;Lee PG;Uchimura T;Seufert CR;Kwon H;Zeng L
肌肉是靠近软骨组织的组织之一。虽然有人认为肌肉可以通过收缩产生机械力来影响骨骼发育，但关于肌肉细胞是否释放生化信号来调节软骨基因表达却知之甚少。我们通过分析在二维或三维条件下与肌肉细胞共培养的软骨细胞，验证了肌肉细胞直接调节软骨基质生成的假设。我们发现用C2C12肌细胞培养的软骨细胞阿利新蓝染色增强，胶原II和胶原IX蛋白表达升高。虽然非肌肉细胞不促进软骨基质的产生，但将它们转化为肌肉细胞可增强其促软骨生成活性。此外，肌肉细胞条件培养基导致软骨基质生成增加，表明肌肉细胞分泌促软骨生成因子。综上所述，我们的研究表明肌肉细胞可能在调节软骨基因表达方面发挥重要作用。这一结果可能最终导致发现调节软骨形成和体内平衡的新因素，并为改进软骨再生策略提供见解。
Muscle is one of the tissues located in close proximity to cartilage tissue. Although it has been suggested that muscle could influence skeletal development through generating mechanical forces by means of contraction, very little is known regarding whether muscle cells release biochemical signals to regulate cartilage gene expression. We tested the hypothesis that muscle cells directly regulate cartilage matrix production by analyzing chondrocytes co-cultured with muscle cells in 2D or 3D conditions. We found that chondrocytes cultured with C2C12 muscle cells exhibited enhanced alcian blue staining and elevated expression of collagen II and collagen IX proteins. While non-muscle cells do not promote cartilage matrix production, converting them into muscle cells enhanced their pro-chondrogenic activity. Furthermore, muscle cell-conditioned medium led to increased cartilage matrix production, suggesting that muscle cells secrete pro-chondrogenic factors. Taken together, our study suggests that muscle cells may play an important role in regulating cartilage gene expression. This result may ultimately lead to the discovery of novel factors that regulate cartilage formation and homeostasis, and provide insights into improving the strategies for regenerating cartilage.
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DOI：
10.1073/pnas.0407510102
发表时间：
2005-02-15
影响因子：
11.1
作者：
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通讯作者：
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影响因子：
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影响因子：
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作者：
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通讯作者：
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