A new social gene in Dictyostelium discoideum, chtB.
A new social gene in Dictyostelium discoideum, chtB.
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DOI：
10.1186/1471-2148-13-4
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发表时间：
2013-01-09
影响因子：
3.4
通讯作者：
Strassmann JE
中科院分区：
文献类型：
--
作者：
Santorelli LA;Kuspa A;Shaulsky G;Queller DC;Strassmann JE
竞争性社会互动在自然界中无处不在，但其遗传基础却难以确定。从真核生物微生物的单基因敲除中可以学到很多东西。突变体可以与亲本竞争，以辨别该特定基因的社会影响。盘形盘基骨虫是一种社会性变形虫，在多细胞子实体的构建过程中表现出合作行为。它具有基因组序列，易于遗传操作，是研究合作遗传基础的良好模式生物。当两株盘状孢子虫混合在一起时，一个作弊的菌株可以通过分化出比柄细胞更多的孢子来利用它的社会伙伴。作弊菌株可以在实验室中产生，也可以在野外发现，基因分析表明，作弊行为可以通过多种途径实现。我们已经描述了敲除突变体chtB，它是从筛选的作弊突变体中分离出来的，这些突变体也能够自己形成正常的子实体。当与亲本菌株细胞按相同比例混合时，chtB突变体几乎贡献了孢子总数的60%。为了做到这一点，chtB细胞抑制野生型细胞成为孢子，从计数和野生型细胞减少孢子前基因cotB的表达可以看出。我们没有发现明显的适应成本（形态学、在液体培养基中加倍的时间、孢子产生和萌发效率）与chtB基因敲除的欺骗能力相关。在这项研究中，我们描述了一个新的基因在盘状孢子虫，chtB，当敲除抑制亲本菌株产生孢子。此外，在实验室条件下，我们没有发现与这种行为相关的任何健康成本。
Competitive social interactions are ubiquitous in nature, but their genetic basis is difficult to determine. Much can be learned from single gene knockouts in a eukaryote microbe. The mutants can be competed with the parent to discern the social impact of that specific gene. Dictyostelium discoideum is a social amoeba that exhibits cooperative behavior in the construction of a multicellular fruiting body. It is a good model organism to study the genetic basis of cooperation since it has a sequenced genome and it is amenable to genetic manipulation. When two strains of D. discoideum are mixed, a cheater strain can exploit its social partner by differentiating more spore than its fair share relative to stalk cells. Cheater strains can be generated in the lab or found in the wild and genetic analyses have shown that cheating behavior can be achieved through many pathways. We have characterized the knockout mutant chtB, which was isolated from a screen for cheater mutants that were also able to form normal fruiting bodies on their own. When mixed in equal proportions with parental strain cells, chtB mutants contributed almost 60% of the total number of spores. To do so, chtB cells inhibit wild type cells from becoming spores, as indicated by counts and by the wild type cells’ reduced expression of the prespore gene, cotB. We found no obvious fitness costs (morphology, doubling time in liquid medium, spore production, and germination efficiency) associated with the cheating ability of the chtB knockout. In this study we describe a new gene in D. discoideum, chtB, which when knocked out inhibits the parental strain from producing spores. Moreover, under lab conditions, we did not detect any fitness costs associated with this behavior.
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影响因子：
11.4
作者：
Foster, Kevin R.;Parkinson, Katie;Thompson, Christopher R. L.
通讯作者：
Thompson, Christopher R. L.
影响因子：
9.2
作者：
Buttery, Neil J.;Rozen, Daniel E.;Thompson, Christopher R. L.
通讯作者：
Thompson, Christopher R. L.
影响因子：
7
作者：
Heid, CA;Stevens, J;Williams, PM
通讯作者：
Williams, PM
DOI：
10.1073/pnas.89.18.8803
发表时间：
1992-09-15
影响因子：
11.1
作者：
KUSPA, A;LOOMIS, WF
通讯作者：
LOOMIS, WF
DOI：
10.1073/pnas.95.16.9360
发表时间：
1998-08-04
影响因子：
11.1
作者：
Ponte, E;Bracco, E;Bozzaro, S
通讯作者：
Bozzaro, S