Reliability of tumor markers, chemokines, and metastasis-related molecules in serum.
Reliability of tumor markers, chemokines, and metastasis-related molecules in serum.
复制标题
血清中肿瘤标记，趋化因子和与转移相关的分子的可靠性。
DOI：
10.1684/ecn.2009.0146
复制
发表时间：
2009-03
影响因子：
2.8
通讯作者：
Lokshin AE
中科院分区：
文献类型：
--
作者：
Linkov F;Gu Y;Arslan AA;Liu M;Shore RE;Velikokhatnaya L;Koenig KL;Toniolo P;Marrangoni A;Yurkovetsky Z;Zeleniuch-Jacquotte A;Lokshin AE
人们越来越关注癌症生物标志物、转移相关分子和趋化因子在各种癌症的发生和发展中可能发挥的作用。然而，很少有研究涉及这些生物标志物在健康个体中的长期可靠性。本研究的目的是调查连续数年献血的健康妇女血清样本中多种蛋白质的时间可靠性。从现有的前瞻性队列中随机选择符合条件的受试者，35名绝经后妇女每年重复就诊2次，30名绝经前妇女每年重复就诊3次。使用多路复用Luminex xMAP™技术测量这些女性中55种血清蛋白的水平，这些血清蛋白代表癌症抗原、趋化因子、血管生成和抗血管生成因子、蛋白酶、脂肪因子、凋亡分子和其他标志物。采用xMAP™方法，高检出率（bb0 60%）和可接受可靠性（类内相关系数，ICCs≥0.55）的生物标志物有：癌症抗原：AFP、ca15 -3、CEA、CA-125、SCC、SAA；生长因子/相关分子：ErbB2、IGFBP-1；蛋白酶和粘附分子：mmp - 1,8,9, sE-selectin，人钾化酶（KLK） 8,10, ICAM-1, VCAM-1，趋化因子：fractalkine， mcp -1,2， RANTES, MIP-1α, MIP-1β, Eotaxin, GRO-α, IP-10；血管生成抑制剂：血管抑制素和内皮抑制素；脂肪因子瘦素和抵抗素；凋亡因子：Fas和其他蛋白质间皮素、髓过氧化物酶（MPO）和PAI-1。其余正在研究的生物标志物要么icc小于0.55，要么检测水平较低（< 60%）。这些癌症抗原包括：CA 19-9、CA 72-4、MICA、S100、TTR、ULBP1、ULBP2、ULBP3；蛋白酶：MMP 2、3、7、12、13；趋化因子：MCP-3、MIF、MIG；脂肪因子：瘦素和抵抗素；凋亡因子：FasL、DR5、Cyfra 21-1；血管生成抑制剂和其他标志物：血栓反应蛋白和热休克蛋白（HSP）总之，在所调查的55个生物标志物中，有34个在60%的样本中可检测到，并且ICC≥0.55，表明使用xMAP™方法进行单一血清测量可用于前瞻性流行病学研究。
There is a growing interest in the role that cancer biomarkers, metastasis-related molecules, and chemokines may play in the development and progression of various cancers. However, few studies have addressed the reliability of such biomarkers in healthy individuals over time. The objective of this study was to investigate the temporal reliability of multiple proteins in serum samples from healthy women who donated blood over successive years. Thirty five, postmenopausal women with two, repeated annual visits, and thirty, premenopausal women with three, repeated annual visits were randomly selected among eligible subjects from an existing, prospective cohort. Multiplexing Luminex xMAP™ technology was used to measure the levels of 55 serum proteins representing cancer antigens, chemokines, angiogenic and anti-angiogenic factors, proteases, adipokines, apoptotic molecules, and other markers in these women. The biomarkers with high detection rates (> 60%) and acceptable reliability (intraclass correlation coefficient, ICCs ≥ 0.55) using xMAP™ method were: cancer antigens: AFP, CA 15-3, CEA, CA-125, SCC, SAA; growth factors/related molecules: ErbB2, IGFBP-1; proteases and adhesion molecules: MMP-1, 8, 9, sE-selectin, human kallikreins (KLK) 8,10, ICAM-1, VCAM-1, chemokines: fractalkine, MCP-1,2, RANTES, MIP-1α, MIP-1β, Eotaxin, GRO-α, IP-10; inhibitors of angiogenesis: angiostatin and endostatin; adipokines leptin and resistin; apoptotic factor: Fas, and other proteins mesothelin, myeloperoxidase (MPO), and PAI-1. The rest of the biomarkers under investigation either had ICCs less than 0.55 or had low levels of detection (< 60%). These included cancer antigens: CA 19-9, CA 72-4, MICA, S100, TTR, ULBP1, ULBP2, ULBP3; proteases: MMP 2, 3, 7, 12, 13; chemokines: MCP-3, MIF, MIG; adipokines: leptin and resistin; apoptotic factors: FasL, DR5, Cyfra 21-1; and inhibitors of angiogenesis and other markers: thrombospondin and heat shock protein (HSP) 27. In conclusion, 34 out of the 55 biomarkers investigated were present in detectable levels in > 60% of the samples, and with an ICC ≥0.55, indicating that a single serum measurement can be used in prospective epidemiological studies using the xMAP™ method.
登录
查看更多内容
影响因子：
3.8
作者：
Linkov, Faina;Lisovich, Alex;Ferris, Robert L.
通讯作者：
Ferris, Robert L.
影响因子：
4.7
作者：
Lokshin, Anna E.;Winans, Mathew;Gorelik, Elieser
通讯作者：
Gorelik, Elieser
影响因子：
2.7
作者：
Haim, M;Tanne, D;Behar, S
通讯作者：
Behar, S
DOI：
10.1186/1477-7827-1-49
发表时间：
2003-06-18
期刊：
Reproductive biology and endocrinology : RB&E
影响因子：
--
作者：
Arslan, Alan A;Zeleniuch-Jacquotte, Anne;Toniolo, Paolo
通讯作者：
Toniolo, Paolo
影响因子：
6.8
作者：
Weikert, Cornelia;Westphal, Sabine;Pischon, Tobias
通讯作者：
Pischon, Tobias