Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense.
Genome-Wide Identification and Functional Analyses of the CRK Gene Family in Cotton Reveals GbCRK18 Confers Verticillium Wilt Resistance in Gossypium barbadense.
复制标题
棉花 CRK 基因家族的全基因组鉴定和功能分析揭示 GbCRK18 赋予海岛棉黄萎病抗性。
DOI：
10.3389/fpls.2018.01266
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发表时间：
2018
影响因子：
5.6
通讯作者：
Dai XF
中科院分区：
文献类型：
--
作者：
Li TG;Zhang DD;Zhou L;Kong ZQ;Hussaini AS;Wang D;Li JJ;Short DPG;Dhar N;Klosterman SJ;Wang BL;Yin CM;Subbarao KV;Chen JY;Dai XF
富含半胱氨酸的受体样激酶（CRKs）是植物受体样激酶的一个大亚家族，在植物抗病中起着关键作用。然而，关于棉花中CRK基因家族及其对黄萎病（Verticillium wilt， VW）的作用，人们还缺乏相关知识。黄萎病是由大水花黄萎病（Verticillium dahliae）引起的破坏性疾病，可显著降低棉花产量。在这项研究中，我们鉴定了巴贝登棉基因组（GbCRKs）中总共30个典型的CRKs。其中11个（约占30%）位于A06和D06染色体上，18个由编码在A和D亚基因组中的9个同源对组成。系统发育分析表明，gbcrk可分为四大类，其扩展可能是由串联复制驱动的。抗性和易感棉花品种的GbCRK基因表达谱显示，9个GbCRK基因在大丽花侵染下表达上调。病毒诱导的这9种GbCRKs的基因沉默分别表明，GbCRK18的沉默足以降低巴氏革兰氏菌对VW的抗性。GbCRK18可被大丽花v感染或茉莉酸诱导表达，并表现出质膜定位。因此，我们的表达分析表明，CRK基因家族在黄萎病感染反应中受到差异调控，而基因沉默实验显示，GbCRK18尤其赋予巴氏白藜芦醇抗性。
Cysteine-rich receptor-like kinases (CRKs) are a large subfamily of plant receptor-like kinases that play a critical role in disease resistance in plants. However, knowledge about the CRK gene family in cotton and its function against Verticillium wilt (VW), a destructive disease caused by Verticillium dahliae that significantly reduces cotton yields is lacking. In this study, we identified a total of 30 typical CRKs in a Gossypium barbadense genome (GbCRKs). Eleven of these (>30%) are located on the A06 and D06 chromosomes, and 18 consisted of 9 paralogous pairs encoded in the A and D subgenomes. Phylogenetic analysis showed that the GbCRKs could be classified into four broad groups, the expansion of which has probably been driven by tandem duplication. Gene expression profiling of the GbCRKs in resistant and susceptible cotton cultivars revealed that a phylogenetic cluster of nine of the GbCRK genes were up-regulated in response to V. dahliae infection. Virus-induced gene silencing of each of these nine GbCRKs independently revealed that the silencing of GbCRK18 was sufficient to compromise VW resistance in G. barbadense. GbCRK18 expression could be induced by V. dahliae infection or jasmonic acid, and displayed plasma membrane localization. Therefore, our expression analyses indicated that the CRK gene family is differentially regulated in response to Verticillium infection, while gene silencing experiments revealed that GbCRK18 in particular confers VW resistance in G. barbadense.
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影响因子：
14.9
作者：
Hunter S;Jones P;Mitchell A;Apweiler R;Attwood TK;Bateman A;Bernard T;Binns D;Bork P;Burge S;de Castro E;Coggill P;Corbett M;Das U;Daugherty L;Duquenne L;Finn RD;Fraser M;Gough J;Haft D;Hulo N;Kahn D;Kelly E;Letunic I;Lonsdale D;Lopez R;Madera M;Maslen J;McAnulla C;McDowall J;McMenamin C;Mi H;Mutowo-Muellenet P;Mulder N;Natale D;Orengo C;Pesseat S;Punta M;Quinn AF;Rivoire C;Sangrador-Vegas A;Selengut JD;Sigrist CJ;Scheremetjew M;Tate J;Thimmajanarthanan M;Thomas PD;Wu CH;Yeats C;Yong SY
通讯作者：
Yong SY
影响因子：
5.1
作者：
Chen, KG;Du, LQ;Chen, ZX
通讯作者：
Chen, ZX
DOI：
10.1016/j.bbrc.2014.02.013
发表时间：
2014-03-07
影响因子：
3.1
作者：
Idanheimo, Niina;Gauthier, Adrien;Wrzaczek, Michael
通讯作者：
Wrzaczek, Michael
影响因子：
4.5
作者：
Chern M;Xu Q;Bart RS;Bai W;Ruan D;Sze-To WH;Canlas PE;Jain R;Chen X;Ronald PC
通讯作者：
Ronald PC
影响因子：
7.2
作者：
Acharya, Biswa R.;Raina, Surabhi;Raina, Ramesh
通讯作者：
Raina, Ramesh