Selective recording of physiologically evoked neural activity in a mixed autonomic nerve using a minimally invasive array.
Selective recording of physiologically evoked neural activity in a mixed autonomic nerve using a minimally invasive array.
复制标题
作者：
神经调节装置的实时闭环控制需要对周围神经系统的神经活动进行长期监测。虽然存在许多信号提取方法，但很少有方法既能在临床上可行，又能从脆弱的外周内脏神经中提取小信号。在这里，我们报告了我们的微创记录和分析技术从内脏神经提取低信噪比(SNR)神经活动，具有高度特异性的纤维类型和类别。在一个广泛表征的活体模型中，记录了在受控膀胱充盈和排尿过程中生理诱发的大鼠盆腔神经的复杂活动，这为验证我们的技术提供了一个很好的试验台。将乌拉坦麻醉的雄性大鼠(n = 12)植入四电极平面阵列，在膀胱内进行连续流动型膀胱测压，通过记录持续输注生理盐水过程中膀胱压力的变化来测量尿动力学功能。我们证明了差分双极记录和互相关分析提取传入和传出活动，并根据传导速度区分纤维亚群。整合Aδ传入纤维活性与排尿期膀胱压力相关(R2：0.6 6 ± 0.0 6)，而不受辣椒素膀胱内激活伤害性传入纤维的影响(R2：0.5 9 ± 0.14，P = 0.5 4，n = 3)。总而言之，这些结果表明我们的微创记录和分析技术在提取低信噪比/负信噪比的混合神经活动方面具有选择性。此外，整合的传入活动与膀胱压力有可靠的相关性，是开发膀胱控制闭环系统技术的有希望的第一步。
Real-time closed-loop control of neuromodulation devices requires long-term monitoring of neural activity in the peripheral nervous system. Although many signal extraction methods exist, few are both clinically viable and designed for extracting small signals from fragile peripheral visceral nerves. Here, we report that our minimally invasive recording and analysis technology extracts low to negative signal to noise ratio (SNR) neural activity from a visceral nerve with a high degree of specificity for fiber type and class. Complex activity was recorded from the rat pelvic nerve that was physiologically evoked during controlled bladder filling and voiding, in an extensively characterized in vivo model that provided an excellent test bed to validate our technology. Urethane-anesthetized male rats (n = 12) were implanted with a four-electrode planar array and the bladder instrumented for continuous-flow cystometry, which measures urodynamic function by recording bladder pressure changes during constant infusion of saline. We demonstrated that differential bipolar recordings and cross-correlation analyses extracts afferent and efferent activity, and discriminated between subpopulations of fibers based on conduction velocity. Integrated Aδ afferent fiber activity correlated with bladder pressure during voiding (r2: 0.66 ± 0.06) and was not affected by activating nociceptive afferents with intravesical capsaicin (r2: 0.59 ± 0.14, P = 0.54, and n = 3). Collectively, these results demonstrate our minimally invasive recording and analysis technology is selective in extracting mixed neural activity with low/negative SNR. Furthermore, integrated afferent activity reliably correlates with bladder pressure and is a promising first step in developing closed-loop technology for bladder control.
登录
查看更多内容
DOI：
10.3390/s22010058
发表时间：
2021-12-23
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Andreis FR;Metcalfe B;Janjua TAM;Jensen W;Meijs S;Dos Santos Nielsen TGN
通讯作者：
Dos Santos Nielsen TGN
影响因子：
2.5
作者：
HULSEBOSCH, CE;COGGESHALL, RE
通讯作者：
COGGESHALL, RE
影响因子：
1.2
作者：
Dweiri, Yazan M.;Stone, Matthew A.;Durand, Dominique M.
通讯作者：
Durand, Dominique M.
影响因子：
--
作者：
Masi, Emily Battinelli;Levy, Todd;Zanos, Theodoros P
通讯作者：
Zanos, Theodoros P
影响因子：
5
作者：
MAGGI, CA;SANTICIOLI, P;MELI, A
通讯作者：
MELI, A