Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric imaging of synthetic polymer sample spots prepared using ionic liquid matrices.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometric imaging of synthetic polymer sample spots prepared using ionic liquid matrices.
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使用离子液体基质制备的合成聚合物样品点的基质辅助激光解吸/电离飞行时间质谱成像
DOI：
10.1002/rcm.6810
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
S. M. Weidner
中科院分区：
文献类型：
--
作者：
S. J. Gabriel;D. Pfeifer;C. Schwarzinger;U. Panne;S. M. Weidner
通过干燥液滴法制备的基体辅助激光解吸/电离飞行时间质谱（MALDI - TOF MS）的rationalpolymer样品斑点经常显示环状结构，伴随着基体和样品分子以及聚合物同源物本身的可能分离。由于大多数样品斑点都是通过这种简单快速的方法制备的，因此必须找到一种排除这种分离的基质或样品制备方法。方法制备了3种不同的离子液体基质，这些基质以传统的芳香族化合物为基础，用于MALDI - TOF质谱分析。离子液体的形成经核磁共振氢谱证实。采用MALDI成像质谱法监测均匀性。结果离子液体基质具有优良的样品斑点均匀性。斑点可以被采样多次而在质谱上没有可见的差异。没有观察到质谱仪真空中经常观察到的基质损失。减少了必要的激光辐照度，从而减少了聚合物的破碎。结论离子液体基质可以克服传统MALDI干燥液滴制备的典型缺陷——分离。均匀的样品点易于制备，在MS真空中稳定，从而提高了MALDI的重现性。版权所有©2014 John Wiley & Sons， Ltd。
RATIONALEPolymer sample spots for matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) prepared by the dried‐droplet method often reveal ring formation accompanied by possible segregation of matrix and sample molecules as well as of the polymer homologs itself. Since the majority of sample spots are prepared by this simple and fast method, a matrix or sample preparation method that excludes such segregation has to be found.METHODSThree different ionic liquid matrices based on conventionally used aromatic compounds for MALDI‐TOF MS were prepared. The formation of ionic liquids was proven by1H NMR spectroscopy. MALDI‐Imaging mass spectrometry was applied to monitor the homogeneity.RESULTSOur results show a superior sample spot homogeneity using ionic liquid matrices. Spots could be sampled several times without visible differences in the mass spectra. A frequently observed loss of matrix in the mass spectrometer vacuum was not observed. The necessary laser irradiance was reduced, which resulted in less polymer fragmentation.CONCLUSIONSIonic liquid matrices can be used to overcome segregation, a typical drawback of conventional MALDI dried‐droplet preparations. Homogeneous sample spots are easy to prepare, stable in the MS vacuum and, thereby, improve the reproducibility of MALDI. Copyright © 2014 John Wiley & Sons, Ltd.
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影响因子：
2
作者：
Carda-Broch, S;Berthod, A;Armstrong, DW
通讯作者：
Armstrong, DW
影响因子：
7.4
作者：
Armstrong, DW;Zhang, LK;Gross, ML
通讯作者：
Gross, ML
影响因子：
2.9
作者：
Bonnel, David;Franck, Julien;Fournier, Isabelle
通讯作者：
Fournier, Isabelle
蛋白质组学成像和肾脏。
DOI：
--
发表时间：
2013
期刊：
JN. Journal of Nephrology (Milano. 1992)
影响因子：
--
作者：
F. Magni;M. Lalowski;V. Mainini;M. Marchetti‐Deschmann;C. Chinello;A. Urbani;M. Baumann
通讯作者：
M. Baumann
通过 LC-MALDI-TOF MS 耦合和“MassChrom2D”数据分析测定共聚物组成
影响因子：
2.5
作者：
S. Weidner;J. Falkenhagen;I. Bressler
通讯作者：
I. Bressler