Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome-Metabolomics Analysis.
Gallic Acid Alleviates Gut Dysfunction and Boosts Immune and Antioxidant Activities in Puppies Under Environmental Stress Based on Microbiome-Metabolomics Analysis.
复制标题
DOI：
10.3389/fimmu.2021.813890
复制
发表时间：
2021
影响因子：
7.3
通讯作者：
Deng B
中科院分区：
文献类型：
--
作者：
Yang K;Deng X;Jian S;Zhang M;Wen C;Xin Z;Zhang L;Tong A;Ye S;Liao P;Xiao Z;He S;Zhang F;Deng J;Zhang L;Deng B
生命早期暴露在环境压力下会破坏肠道屏障，导致炎症反应和肠道微生物区系组成的变化。没食子酸(GA)是一种天然植物多酚，因其具有抗氧化、抗炎和抗菌等特性而受到人们的极大关注，这些特性有助于维持肠道健康。为了评价日粮中添加赤霉素A是否能缓解环境应激，将19只幼犬随机分为3组：1)基础日粮(对照组)；2)基础日粮+运输(TS)；3)基础日粮中添加500 mg/kg的GA+运输(TS+GA)。在1周的补充期后，TS组和TS+GA组的幼犬被从一个应激环境转移到另一个宜居的地方，而CON组的幼犬则被留在应激环境中。结果表明，在整个试验期间，GA显著降低了仔犬的腹泻率，运输后血清皮质醇和HSP-70水平也有一定程度的下降。此外，GA还可减轻多种环境应激源引起的氧化应激和炎症反应。同时，饲喂GA的幼犬运输后粪便细菌和乳酸菌丰度较高，变形杆菌、志贺氏菌和严格梭状芽孢杆菌较低。结果表明，TS+GA组的短链脂肪酸总量和醋酸含量最高。此外，粪便和血清代谢组学分析表明，GA显著逆转了应激引起的氨基酸代谢、脂肪代谢、碳水化合物代谢和核苷酸代谢的异常。最后，进行Spearman相关分析，探索微生物区系和代谢物之间的综合关系。总体而言，日粮中补充GA可减轻应激幼犬的氧化应激和炎症反应，这是通过对肠道微生物区系和可能支持肠道和宿主健康的代谢物的有益改变来实现的。
Early-life exposure to environmental stress disrupts the gut barrier and leads to inflammatory responses and changes in gut microbiota composition. Gallic acid (GA), a natural plant polyphenol, has received significant interest for its antioxidant, anti-inflammatory, and antimicrobial properties that support the maintenance of intestinal health. To assess whether dietary supplementation of GA alleviates environmental stress, a total of 19 puppies were randomly allocated to the following three dietary treatments for 2 weeks: 1) basal diet (control (CON)); 2) basal diet + transportation (TS); and 3) basal diet with the addition of 500 mg/kg of GA + transportation (TS+GA). After a 1-week supplementation period, puppies in the TS and TS+GA groups were transported from a stressful environment to another livable location, and puppies in the CON group were then left in the stressful environment. Results indicated that GA markedly reduced the diarrhea rate in puppies throughout the trial period and caused a moderate decline of serum cortisol and HSP-70 levels after transportation. Also, GA alleviated the oxidative stress and inflammatory response caused by multiple environmental stressors. Meanwhile, puppies fed GA had a higher abundance of fecal Firmicutes and Lactobacillus and lower Proteobacteria, Escherichia–Shigella, and Clostridium_sensu_stricto_1 after transportation. As a result, the TS+GA group had the highest total short-chain fatty acids and acetic acid. Also, the fecal and serum metabolomics analyses revealed that GA markedly reversed the abnormalities of amino acid metabolism, lipid metabolism, carbohydrate metabolism, and nucleotide metabolism caused by stresses. Finally, Spearman’s correlation analysis was carried out to explore the comprehensive microbiota and metabolite relationships. Overall, dietary supplementation of GA alleviates oxidative stress and inflammatory response in stressed puppies by causing beneficial shifts on gut microbiota and metabolites that may support gut and host health.
登录
查看更多内容
影响因子：
15.5
作者：
Estaki M;Pither J;Baumeister P;Little JP;Gill SK;Ghosh S;Ahmadi-Vand Z;Marsden KR;Gibson DL
通讯作者：
Gibson DL
DOI：
10.3233/jad-2009-1135
发表时间：
2009
期刊：
Journal of Alzheimer's disease : JAD
影响因子：
--
作者：
Ferruzzi MG;Lobo JK;Janle EM;Cooper B;Simon JE;Wu QL;Welch C;Ho L;Weaver C;Pasinetti GM
通讯作者：
Pasinetti GM
影响因子：
4.2
作者：
Galley JD;Nelson MC;Yu Z;Dowd SE;Walter J;Kumar PS;Lyte M;Bailey MT
通讯作者：
Bailey MT
DOI：
10.3390/ani10112016
发表时间：
2020-11-02
期刊：
Animals : an open access journal from MDPI
影响因子：
--
作者：
Abbas Z;Hu L;Fang H;Sammad A;Kang L;Brito LF;Xu Q;Wang Y
通讯作者：
Wang Y
影响因子：
3.2
作者：
Cai, L.;Li, Y. P.;Jiang, X. R.
通讯作者：
Jiang, X. R.