Exposure to a social stressor disrupts the community structure of the colonic mucosa-associated microbiota.
Exposure to a social stressor disrupts the community structure of the colonic mucosa-associated microbiota.
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DOI：
10.1186/1471-2180-14-189
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发表时间：
2014-07-15
期刊：
影响因子：
4.2
通讯作者：
Bailey MT
中科院分区：
文献类型：
--
作者：
Galley JD;Nelson MC;Yu Z;Dowd SE;Walter J;Kumar PS;Lyte M;Bailey MT
哺乳动物胃肠道（GI）的微生物群由与宿主生理功能相互作用的多种肠道细菌组成。通过抗生素或饮食变化等外源手段使这些种群失调，可能对宿主的健康产生不利影响。来自我们等实验室的研究表明，暴露于心理压力会破坏肠道微生物群的种群分布。到目前为止，这些研究主要集中在长期的应激源（在几天内重复），并评估了粪便细菌种群。目前尚不清楚较短的压力源是否也会影响微生物群，以及结肠粘膜相关人群是否也会受到影响。粘膜相关的微生物群存在于宿主免疫系统的元件附近，两者紧密相关。因此，应强调这些人群的变化。此外，应激源可以诱导不同品系小鼠的焦虑样行为和皮质酮输出的差异反应。因此，还检查了应激物暴露是否可以对近交系C57 BL/6小鼠和远交系CD-1小鼠中的结肠微生物群具有对比效应。在本研究中，我们使用高通量焦磷酸测序来评估C57 BL/6小鼠的结肠粘膜相关微生物概况上的单次2小时暴露于称为社会破坏（SDR）的社会应激源的影响。这些数据表明，暴露于应激源显着改变了社区概况，显着降低了两个属和一个家庭的高度丰富的肠道细菌，包括乳酸杆菌属的相对比例。使用定量实时聚合酶链反应（qPCR）技术证实了这一发现。使用qPCR还鉴定了细菌丰度的小鼠品系特异性差异。L. reuteri，一种免疫调节物种，在应激暴露的CD-1小鼠中减少，但C57 BL/6小鼠没有。这些数据表明，应激暴露可以影响微生物种群，包括乳酸杆菌，这是密切相关的结肠粘膜。由于乳酸杆菌对人类健康有有益的影响，因此压力引起的乳酸杆菌数量减少可能对健康产生重要影响。
The microbiota of the mammalian gastrointestinal (GI) tract consists of diverse populations of commensal bacteria that interact with host physiological function. Dysregulating these populations, through exogenous means such as antibiotics or dietary changes, can have adverse consequences on the health of the host. Studies from laboratories such as ours have demonstrated that exposure to psychological stressors disrupts the population profile of intestinal microbiota. To date, such studies have primarily focused on prolonged stressors (repeated across several days) and have assessed fecal bacterial populations. It is not known whether shorter stressors can also impact the microbiota, and whether colonic mucosa-associated populations can also be affected. The mucosa-associated microbiota exist in close proximity to elements of the host immune system and the two are tightly interrelated. Therefore, alterations in these populations should be emphasized. Additionally, stressors can induce differential responses in anxiety-like behavior and corticosterone outputs in variant strains of mice. Thus, whether stressor exposure can have contrasting effects on the colonic microbiota in inbred C57BL/6 mice and outbred CD-1 mice was also examined. In the present study, we used high throughput pyrosequencing to assess the effects of a single 2-hour exposure to a social stressor, called social disruption (SDR), on colonic mucosa-associated microbial profiles of C57BL/6 mice. The data indicate that exposure to the stressor significantly changed the community profile and significantly reduced the relative proportions of two genera and one family of highly abundant intestinal bacteria, including the genus Lactobacillus. This finding was confirmed using a quantitative real-time polymerase chain reaction (qPCR) technique. The use of qPCR also identified mouse strain-specific differences in bacterial abundances. L. reuteri, an immunomodulatory species, was decreased in stressor-exposed CD-1 mice, but not C57BL/6 mice. These data illustrate that stressor exposure can affect microbial populations, including the lactobacilli, that are closely associated with the colonic mucosa. Because the lactobacilli can have beneficial effects on human health, stressor-induced reductions of their population could have important health implications.
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DOI：
10.1126/science.1198469
发表时间：
2011-01-21
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Atarashi K;Tanoue T;Shima T;Imaoka A;Kuwahara T;Momose Y;Cheng G;Yamasaki S;Saito T;Ohba Y;Taniguchi T;Takeda K;Hori S;Ivanov II;Umesaki Y;Itoh K;Honda K
通讯作者：
Honda K
影响因子：
3.7
作者：
Bangsgaard Bendtsen KM;Krych L;Sørensen DB;Pang W;Nielsen DS;Josefsen K;Hansen LH;Sørensen SJ;Hansen AK
通讯作者：
Hansen AK
影响因子：
3.7
作者：
Barouei J;Moussavi M;Hodgson DM
通讯作者：
Hodgson DM
影响因子：
2.9
作者：
Cuevas, Marielly;Flores, Idhaliz;Appleyard, Caroline B.
通讯作者：
Appleyard, Caroline B.
影响因子：
2.7
作者：
Bouwknecht, J. Adriaan;Paylor, Richard
通讯作者：
Paylor, Richard