Isotopically Dimethyl Labeling-Based Quantitative Proteomic Analysis of Phosphoproteomes of Soybean Cultivars.
Isotopically Dimethyl Labeling-Based Quantitative Proteomic Analysis of Phosphoproteomes of Soybean Cultivars.
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基于同位素二甲基标记的大豆品种磷酸化蛋白质组的定量蛋白质组分析
DOI：
10.3390/biom11081218
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发表时间：
2021-08-16
期刊：
影响因子：
5.5
通讯作者：
Li N
中科院分区：
文献类型：
--
作者：
Moradi A;Dai S;Wong EOY;Zhu G;Yu F;Lam HM;Wang Z;Burlingame A;Lin C;Afsharifar A;Yu W;Wang T;Li N
应用同位素标记技术对两个不同大豆品种干旱反应中的磷蛋白组学变化进行了定量的翻译后修饰(PTM)蛋白质组学研究。共鉴定出9457个磷酸肽，对应4571个磷酸化蛋白基团和3889个主要磷化蛋白，包含9个由279个蛋白组成的蛋白家族。这些磷蛋白共含有8087个亚磷酸盐，其中6106个是新鉴定的，占目前大豆亚磷酸盐资源库的54%。通过生物信息学分析，这些磷酸酶被转化为高度保守的激酶对接位点，预测了6个与新发现的9个激酶家族相匹配的激酶家族。过度翻译后修饰蛋白(OPP)占这些主要磷酸蛋白的2.1%。其中大部分是光感受器、mRNA结合蛋白、组蛋白结合蛋白、磷脂结合蛋白以及蛋白激酶/磷酸酶。亚群总体分布符合指数衰减规律，Y=4.13e−0.098X−0.04。在218个显著调控的独特磷酸肽组中，有188个磷酸蛋白在失水条件下受耐旱品种的调控。这些受显著调控的磷蛋白(SRP)主要集中在水分运输和剥夺、蛋氨酸代谢过程、光合作用/光反应、对镉离子的响应、渗透胁迫和ABA反应等生物学功能中。17个SRP和15个SRP分别是蛋白激酶/磷酸酶和转录因子。生物信息学分析再次揭示了GmSRK2I、GmCIPK25和GmAKINCAMK1这三个钙依赖蛋白激酶家族的成员组成了一个磷信号转导网络，调节着这个耐旱品种的离子通道活性和许多核事件，这可能与大豆在缺水条件下的抗旱性有关。
Isotopically dimethyl labeling was applied in a quantitative post-translational modification (PTM) proteomic study of phosphoproteomic changes in the drought responses of two contrasting soybean cultivars. A total of 9457 phosphopeptides were identified subsequently, corresponding to 4571 phosphoprotein groups and 3889 leading phosphoproteins, which contained nine kinase families consisting of 279 kinases. These phosphoproteins contained a total of 8087 phosphosites, 6106 of which were newly identified and constituted 54% of the current soybean phosphosite repository. These phosphosites were converted into the highly conserved kinase docking sites by bioinformatics analysis, which predicted six kinase families that matched with those newly found nine kinase families. The overly post-translationally modified proteins (OPP) occupies 2.1% of these leading phosphoproteins. Most of these OPPs are photoreceptors, mRNA-, histone-, and phospholipid-binding proteins, as well as protein kinase/phosphatases. The subgroup population distribution of phosphoproteins over the number of phosphosites of phosphoproteins follows the exponential decay law, Y = 4.13e−0.098X − 0.04. Out of 218 significantly regulated unique phosphopeptide groups, 188 phosphoproteins were regulated by the drought-tolerant cultivar under the water loss condition. These significantly regulated phosphoproteins (SRP) are mainly enriched in the biological functions of water transport and deprivation, methionine metabolic processes, photosynthesis/light reaction, and response to cadmium ion, osmotic stress, and ABA response. Seventeen and 15 SRPs are protein kinases/phosphatases and transcription factors, respectively. Bioinformatics analysis again revealed that three members of the calcium dependent protein kinase family (CAMK family), GmSRK2I, GmCIPK25, and GmAKINβ1 kinases, constitute a phosphor-relay-mediated signal transduction network, regulating ion channel activities and many nuclear events in this drought-tolerant cultivar, which presumably contributes to the development of the soybean drought tolerance under water deprivation process.
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影响因子：
20.5
作者：
Boudsocq, Marie;Sheen, Jen
通讯作者：
Sheen, Jen
影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
影响因子：
3.3
作者：
Buendig, Christin;Jozefowicz, Anna Maria;Winkelmann, Traud
通讯作者：
Winkelmann, Traud
影响因子：
8.6
作者：
Furusawa, C;Kaneko, K
通讯作者：
Kaneko, K
影响因子：
5.1
作者：
Abrahám, E;Rigó, G;Szabados, L
通讯作者：
Szabados, L