Platelet electrical resistance for measuring platelet activation and adhesion in human health and disease.
Platelet electrical resistance for measuring platelet activation and adhesion in human health and disease.
复制标题
用于测量人体健康和疾病中的血小板活化和粘附性的血小板电阻。
DOI：
10.1016/j.thromres.2020.12.012
复制
发表时间：
2021-03
影响因子：
7.5
通讯作者：
Campbell RA
中科院分区：
文献类型：
--
作者：
Griffiths TM;Page L;Weyrich AS;Rondina MT;Campbell RA
测量血小板反应性变化的能力对于确定血小板生物学的新方面和开发靶向治疗以防止出血或血栓形成非常重要。目前的血小板功能测试允许一次分析一种激动剂。使用多种激动剂和黏附底物在一次实验中对血小板进行表型的能力可以比目前的方法更深入地了解改变的途径。我们推测，血小板电阻(PER)可用于更全面的血小板表型。血小板来自健康男性和女性供者(年龄39.6±6.9)和败血症患者(年龄44.0±13.5)。PER-96孔板被涂有包括纤维蛋白原和胶原在内的各种底物。在凝血酶或惊厥毒素存在或不存在的情况下，将血小板添加到包被的平板中。通过测量电阻抗的变化来监测血小板的激活和铺展。血小板与纤维蛋白原和胶原的黏附增加了阻抗。此外，对凝血酶或惊厥毒素的反应增加了阻抗。在没有血小板或孔洞没有涂层的情况下，没有观察到阻抗的变化，这表明阻抗的变化直接是由于血小板的黏附和激活。当以纤维蛋白原为底物时，抑制整合素αIIbβ3可降低阻抗，这与血小板依赖效应一致。与健康献血者相比，败血症患者的血小板引起的阻抗增加，表明该方法可用于评估血小板的高反应性。PER可作为高通量工具用于测量健康和疾病中的血小板反应性，在这些疾病中，血小板活化增加。
The ability to measure changes in platelet reactivity is important to identify novel aspects of platelet biology and develop targeted therapeutics to prevent bleeding or thrombosis. Current platelet function testing allows for single agonist analysis at a time. The ability to phenotype platelets in a single assay with multiple agonists and adhesion substrates could yield more insights into altered pathways than are feasible with current approaches. We hypothesized platelet electrical resistance (PER) could be used for more comprehensive phenotyping of platelets. Platelets were isolated from male and female healthy donors (age 39.6±6.9) and septic patients (age 44.0±13.5). PER 96-well plates were coated with various substrates, including fibrinogen and collagen. Platelets were added to the coated plates in the presence or absence of thrombin or convulxin. Platelet activation and spreading was monitored by measuring changes in electrical impedance. Platelets adhesion to fibrinogen and collagen increased impedance. In addition, impedance increased in response to thrombin or convulxin. No changes in impedance were observed in the absence of platelets or when wells were uncoated, indicating changes in impedance were directly due to platelet adhesion and activation. Inhibiting integrin αIIbβ3 decreased impedance when fibrinogen was used as a substrate, consistent with platelet-dependent effects. Platelets from septic patients caused increased impedance compared to healthy donors, demonstrating this assay can be used to assess platelet hyperreactivity. PER can be applied as a high throughput tool to measure platelet reactivity in health and disease, where platelet activation is increased.
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DOI：
10.1007/978-1-4939-7196-1_24
发表时间：
2017-01-01
期刊：
HEMOSTASIS AND THROMBOSIS
影响因子：
--
作者：
Hvas, Anne-Mette;Grove, Erik Lerkevang
通讯作者：
Grove, Erik Lerkevang
DOI：
10.1177/2211068214561025
发表时间：
2015-04
期刊：
Journal of laboratory automation
影响因子：
--
作者：
Srinivasan B;Kolli AR;Esch MB;Abaci HE;Shuler ML;Hickman JJ
通讯作者：
Hickman JJ
影响因子：
20.1
作者：
Rondina, Matthew T.;Voora, Deepak;Rowley, Jesse W.
通讯作者：
Rowley, Jesse W.
影响因子：
2.8
作者：
Michelson, Alan D.
通讯作者：
Michelson, Alan D.
DOI：
10.1111/j.1538-7836.2011.04208.x
发表时间：
2011-04
期刊：
Journal of thrombosis and haemostasis : JTH
影响因子：
--
作者：
Rondina MT;Schwertz H;Harris ES;Kraemer BF;Campbell RA;Mackman N;Grissom CK;Weyrich AS;Zimmerman GA
通讯作者：
Zimmerman GA