Prevalence and Relatedness of mcr-1-Mediated Colistin-Resistant Escherichia coli Isolated From Livestock and Farmers in Japan.
Prevalence and Relatedness of mcr-1-Mediated Colistin-Resistant Escherichia coli Isolated From Livestock and Farmers in Japan.
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DOI：
10.3389/fmicb.2021.664931
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发表时间：
2021
影响因子：
5.2
通讯作者：
Yano H
中科院分区：
文献类型：
--
作者：
Nakano A;Nakano R;Nishisouzu R;Suzuki Y;Horiuchi S;Kikuchi-Ueda T;Ubagai T;Ono Y;Yano H
粘菌素用于治疗人类和牲畜的传染病;它也被用作牲畜的饲料添加剂约50年。mcr-1质粒介导的粘菌素耐药基因自2015年在中国发现以来，在全球范围内均有检测到，主要在家畜中发现。在这项研究中，我们调查了日本家畜和农民中mcr介导的粘菌素耐药大肠杆菌的患病率和特征。2013年至2015年，我们从72个畜牧场（58个养牛场和14个养猪场）的295头健康牲畜（202头牛和93头猪）和62名健康农民收集了粪便样本。28株携带E.从25头牲畜（6头牛和19头猪）和3名农民（2名养牛农民和1名养猪农民）中分离出大肠杆菌菌株。携带mcr-1的E.家畜和农民大肠杆菌检出率分别为8.47%和4.84%。其中3株耐药基因可通过mcr-1质粒转移到大肠杆菌中。coli J53在低频（10−7-10−8）。6株菌株共携带mcr-1和CTX-M β-内酰胺酶（CTX-M-14、CTX-M-27或CTX-M-156）。从4个农场（农场C，I，N和P）的牲畜和农民获得的分离株，9株具有相同的基因型特征（序列类型和脉冲场凝胶电泳带型），质粒特征（不相容性组和质粒转移性），和最低抑菌浓度。因此，研究结果表明，克隆菌株可以在农场内的牲畜和农民中传播。据我们所知，这是第一项检测mcr-1介导的粘菌素耐药大肠杆菌的克隆相关性的研究。牲畜和农民的大肠杆菌。这表明农民获得携带mcr-1菌株的风险较高，呼吁我们基于同一健康理念予以关注。
Colistin is used to treat infectious diseases in humans and livestock; it has also been used as a feed additive for livestock for approximately 50 years. Since the mcr-1 plasmid-mediated colistin resistance gene was discovered in China in 2015, it has been detected worldwide, mainly in livestock. In this study, we investigated the prevalence and characteristics of mcr-mediated colistin-resistant Escherichia coli in livestock and farmers in Japan. We collected fecal samples from 295 healthy livestock (202 cattle and 93 swine) and 62 healthy farmers from 72 livestock farms (58 cattle farms and 14 swine farms) between 2013 and 2015. Twenty-eight mcr-1-harboring E. coli strains were isolated from 25 livestock (six cattle and 19 swine) and three farmers (two cattle farmers and one swine farmer). The prevalence rates of mcr-1-harboring E. coli in livestock and farmers were 8.47 and 4.84%, respectively. Of the 28 strains, the resistance genes of three were transferable via the mcr-1-coding plasmids to E. coli J53 at low frequencies (10−7–10−8). Six strains coharbored mcr-1 with CTX-M β-lactamases (CTX-M-14, CTX-M-27, or CTX-M-156). Of the isolates obtained from livestock and farmers in four farms (farms C, I, N, and P), nine strains had the same genotypical characteristics (sequence types and pulsed-field gel electrophoresis band patterns), plasmid characteristics (incompatibility group and plasmid transferability), and minimum inhibitory concentrations. Thus, the findings suggested that clonal strains could spread among livestock and farmers within farms. To our knowledge, this is the first study to detect clonal relatedness of mcr-1-mediated colistin-resistant E. coli in livestock and farmers. It is suggested that farmers are at a higher risk of acquiring mcr-1-harboring strains, calling for our attention based on the One Health concept.
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影响因子：
5.2
作者：
Dorado-Garcia, Alejandro;Smid, Joost H.;Heederik, Dick J. J.
通讯作者：
Heederik, Dick J. J.
影响因子：
5.2
作者：
Hong, Jun Sung;Song, Wonkeun;Jeong, Seok Hoon
通讯作者：
Jeong, Seok Hoon
DOI：
10.1016/j.ijantimicag.2017.11.015
发表时间：
2018-04-01
影响因子：
10.8
作者：
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通讯作者：
Chen, Sheng
影响因子：
3.2
作者：
Khine NO;Lugsomya K;Kaewgun B;Honhanrob L;Pairojrit P;Jermprasert S;Prapasarakul N
通讯作者：
Prapasarakul N
影响因子：
5.2
作者：
Dallenne, Caroline;Da Costa, Anaelle;Arlet, Guillaume
通讯作者：
Arlet, Guillaume