A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.
A high omega-3 fatty acid diet has different effects on early and late stage myeloid progenitors.
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DOI：
10.1007/s11745-010-3491-3
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发表时间：
2011-01
期刊：
影响因子：
1.9
通讯作者：
Sollars VE
中科院分区：
文献类型：
--
作者：
Varney ME;Buchanan JT;Dementieva Y;Hardman WE;Sollars VE
多不饱和ω-3（n-3）和ω-6（n-6）脂肪酸（FA）对造血的作用是复杂的，因为这两种FA形式都被加工成白三烯、类花生酸和胡萝卜素，它们可以具有独立的作用。这些FA具有拮抗作用，因为n-6 FA胡萝卜素倾向于促增殖和促炎，而n-3 FA胡萝卜素的作用相反。我们先前已经证明，高n-3 FA饮食减少了FVB X sv 129 F1杂交小鼠中晚期髓系祖细胞室的大小。为了测定高n-3 FA饮食对骨髓生成早期阶段的影响，我们喂养C57 BL/6 J小鼠高n-3 FA饮食或与西方饮食相似的n-3/n-6 FA水平，并用流式细胞术和集落形成细胞测定法测定对骨髓生成的影响。结果表明，在高n-3 FA饮食中，普通髓系祖细胞区室的扩增，其不会持续到后期阶段，在后期阶段中，在高n-3 FA喂养的动物中祖细胞的数量实际上较低。对造血干细胞系EML-克隆1的体外研究表明，用二十碳五烯酸（n-3 FA）或花生四烯酸（n-6 FA）培养的细胞在细胞活力方面没有差异，但花生四烯酸更快地产生具有低水平巨噬细胞发育标志物F4/80的祖细胞。
The effects of the polyunsaturated omega-3 (n-3) and omega-6 (n-6) fatty acids (FA) on hematopoiesis are complex in that both FA forms are processed into leukotrienes, eicosanoids, and prostaglandins, which can have independent effects. These FA have antagonistic effects in that n-6 FA prostaglandins tend to be pro-proliferative and pro-inflammatory, while the effects of n-3 FA prostaglandins are the opposite. We have previously shown that diets high in n-3 FA reduce the size of the middle to later stage myeloid progenitor compartment in FVB X sv129 F1hybrid mice. To assay the effects of high n-3 FA diets on earlier stages of myelopoiesis, we fed C57BL/6J mice diets high in n-3 FA or levels of n-3/n-6 FA similar to western diets and assayed the effects on myelopoiesis with flow cytometry and colony forming cell assays. Results indicate an expansion of the common myeloid progenitor cell compartment in high n-3 FA diets, which does not persist into later stages where the number of progenitor cells is actually lower in high n-3 FA fed animals. Investigations in vitro with the hematopoietic stem cell line EML-clone 1 indicate that cells cultured with eicosapentaenoic acid (n-3 FA) or arachidonic acid (n-6 FA) have no differences in cell viability but that arachidonic acid more rapidly produces progenitors with low levels of the macrophage developmental marker, F4/80.
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影响因子：
64.5
作者：
Goessling W;North TE;Loewer S;Lord AM;Lee S;Stoick-Cooper CL;Weidinger G;Puder M;Daley GQ;Moon RT;Zon LI
通讯作者：
Zon LI
影响因子：
4.4
作者：
Kelley, DS
通讯作者：
Kelley, DS
影响因子：
4.3
作者：
Lord, Allegra M.;North, Trista E.;Zon, Leonard I.
通讯作者：
Zon, Leonard I.
影响因子：
4.5
作者：
Varney, Melinda E.;Hardman, W. Elaine;Sollars, Vincent E.
通讯作者：
Sollars, Vincent E.
影响因子：
2.9
作者：
Hardman, W. Elaine;Ion, Gabriela
通讯作者：
Ion, Gabriela